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pKO403_lacZ_Cm

Plasmid

#174725

Purpose

Bifidobacterium-E.coli shuttle vector for seamless gene cloning

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

Type

Empty backbone

Expression

Bacterial

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDAS12222_U6-pegRNA-BFP

Plasmid

#177181

Purpose

pegRNA cloning plasmid with BFP marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB

Plasmid

#132929

Purpose

Minimal HIV-1 packaging plasmid for gag and pol expression

Depositor

Jeremy Luban

Article

Berthoux et al J Virol. 2004 Nov;78(21):11739-50. doi:

Insert

gag and pol

Use

Lentiviral

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSAM_Cam

Plasmid

#91570

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

Depositor

Harry Mobley

Article

Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only
pGR-L3S2P21

Plasmid

#46004

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P21 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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7TG

Plasmid

#24314

Depositor

Roel Nusse

Article

Brugmann et al Development. 2007 Sep . 134(18):3283-95.

Insert

7xTcf-eGFP

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-Cre

Plasmid

#235151

Purpose

Expresses Cre recombinase under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

Cre

Use

AAV

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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mTFP1-pBAD

Plasmid

#54553

Purpose

Localization: Protein Expression Vector , Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

Type

Empty backbone

Tags

6xHis and mTFP1

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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HT-mGold

Plasmid

#183986

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

HaloTag-mGold

Tags

HaloTag, mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Article

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLCP FLAG B55 alpha

Plasmid

#13804

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha Flag (PPP2R2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pQCXIN-TetR-mCherry

Plasmid

#59417

Purpose

retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repressor protein (TetR)

Use

Retroviral

Tags

mCherry

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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p52sheLL

Plasmid

#46950

Purpose

HPV52 L1 and L2 Bicistronic, too large to self-package

Depositor

John Schiller

Article

Kondo et al J Med Virol. 2008 May;80(5):841-6. doi:

Insert

HPV52L1+L2 (L1, L2 Human)

Expression

Mammalian

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-sgCDKN1B#2 BFP

Plasmid

#60905

Purpose

A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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HA-RNF126

Plasmid

#138647

Purpose

Mammalian expression of 2HA-tagged RNF126

Depositor

Jane McGlade

Article

Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

Insert

RNF126 (RNF126 Mouse)

Tags

HA-HA

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-Zeo

Plasmid

#75088

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID pRetrox

Plasmid

#155203

Purpose

For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

Depositor

Kyle Roux

Article

May et al Cells. 2020 Apr 25;9(5). pii: cells90510

Insert

3xHA-TurboID

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Promoter

tight TRE promotor

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCPP5371

Plasmid

#128732

Purpose

Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag

Depositor

Alan Collmer

Article

Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Puromycin-mVenus-MAP

Plasmid

#44118

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Tags

mVenus-MAP

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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