We narrowed to 56,173 results for: plasmid

1. SJZ6-HIS5

   Plasmid

   #228259

   Purpose

   Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   his5
   

   Expression

   Yeast

   Promoter

   TEF1 S.c.

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SJZ2-TRP1

   Plasmid

   #228256

   Purpose

   Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   TRP1
   

   Expression

   Yeast

   Promoter

   BBC1 S.c.

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SJZ10-LEU2

   Plasmid

   #228262

   Purpose

   Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   LEU2
   

   Expression

   Yeast

   Promoter

   LEU2 K.l.

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS416 CUP1pro-Htt103QdeltaP125-134-YFP

   Plasmid

   #224882

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP125-134 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP125-134

   Promoter

   CUP1

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS416 CUP1pro-Htt103QdeltaP135-170-YFP

   Plasmid

   #224878

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP135-170 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP135-170

   Promoter

   CUP1

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS416 CUP1pro-Htt103QdeltaP141-160-YFP

   Plasmid

   #224886

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP141-160 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP141-160

   Promoter

   CUP1

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS416 CUP1pro-Htt103QdeltaP125-160-YFP

   Plasmid

   #224883

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP125-160 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP125-160

   Promoter

   CUP1

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS416 CUP1pro-Htt103QdeltaP141-170-YFP

   Plasmid

   #224880

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP141-170 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP141-170

   Promoter

   CUP1

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS416 CUP1pro-Htt103QdeltaP161-170-YFP

   Plasmid

   #224876

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   HTT103QdeltaP161-170 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   delta161-170

   Promoter

   CUP1

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SOX2-3'gRNA

    Plasmid

    #215543

    Purpose

    gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid

    #215544

    Purpose

    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

    Depositor

    Kai Wang

    Insert

    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    

    Use

    CRISPR

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGU06

    Plasmid

    #212148

    Purpose

    Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.

    Depositor

    Kang Zhou

    Insert

    alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Agx1: S. cerevisiae; alaA, tyrB: E. coli, Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    PgyrA

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYCTK010

    Plasmid

    #176714

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania africana (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Ka
    

    Use

    Synthetic Biology

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYCTK006

    Plasmid

    #176710

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter SST2 (response part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    P-SST2
    

    Use

    Synthetic Biology

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYCTK001

    Plasmid

    #176705

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter DIG2 of S. cerevisiae (response part)

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    P-DIG2
    

    Use

    Synthetic Biology

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MDKA-c014

    Plasmid

    #175464

    Purpose

    Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc.

    Depositor

    Opher Gileadi

    Insert

    MDK (MDK Human)
    

    Tags

    HRV 3C-Fc-His6 and PTPRS (Gg) SP

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYCTK007

    Plasmid

    #176711

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter YPS1 (response part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    P-YPS1
    

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYCTK013

    Plasmid

    #176717

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Lachancea fermentati (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Lf
    

    Use

    Synthetic Biology

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYCTK016

    Plasmid

    #176720

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Saccharomyces cerevisiae (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Sc
    

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYCTK021

    Plasmid

    #176725

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Kazachstania africana (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Ka
    

    Use

    Synthetic Biology

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge