We narrowed to 15,153 results for: RING;

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pmGFP-P2A-K(AAA)12-P2A-RFP

Plasmid

#105687

Purpose

Double fluorescent stalling reporter K(AAA)12

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)12
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHsp70-Cas9

Plasmid

#45945

Purpose

The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

3x FLAG

Mutation

Silent change from C to A at bp 834 of insert

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdKatushka2-WPRE

Plasmid

#193337

Purpose

Expresses tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPIPL12-FnCas12a

Plasmid

#199563

Purpose

Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

Fncas12a

Use

CRISPR and Synthetic Biology; Tetracycline induci…

Expression

Bacterial

Promoter

COtetR-PIPL12

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

Plasmid

#85436

Purpose

Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab and anti-CTLA4 scFv

Use

Lentiviral

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only
pET-FleBt

Plasmid

#149302

Purpose

"Enhances the yields of soluble target proteins in E. coli expression system", "Enable quick and efficient protein purification with high purity and stability"

Depositor

Michael Kolbe

Article

Wang et al PLoS Pathog. 2021 Nov 17;17(11):e1010052

Type

Empty backbone

Tags

FleBt and HRV 3C protease recognition site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHiUGE HA-epitope donor ORF0

Plasmid

#200371

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE HA-epitope donor ORF1

Plasmid

#200372

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Expression

Mammalian

Mutation

NA

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKL2013

Plasmid

#172182

Purpose

A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

Depositor

Kan Wang

Article

McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

Insert

mCherry

Use

CRISPR

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEn-Chimera

Plasmid

#61432

Purpose

Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb719-HO

Plasmid

#141200

Purpose

Expresses mRhubarb719 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb719
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-mNINJ1-GFP

Plasmid

#208775

Purpose

Inducibly expresses mouse NINJ1 tagged with GFP

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

mNINJ1 (Ninj1 Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp7

Plasmid

#163591

Purpose

Expresses BMP-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp7 (Bmp7 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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placZα-tdMCP

Plasmid

#191132

Purpose

Expression of tdMCP from a lacZα inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

dMCP_1

Use

Synthetic Biology

Promoter

lac-inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_A0101

Plasmid

#90998

Purpose

Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_dSV40-Aurora A-GFP

Plasmid

#67924

Purpose

Expresses Aurora A-GFP in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

GFP-Aurora A (AURKA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLVE-eGFP

Plasmid

#52581

Purpose

Lentiviral transfer vector driving expression of eGFP in mammalian cells

Depositor

Charles Gersbach

Article

Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pHiUGE HA-epitope donor ORF2

Plasmid

#200373

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15

Plasmid

#176212

Purpose

Bacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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