We narrowed to 58,593 results for: plasmid
-
Plasmid#158990PurposeVector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cellsDepositorInsertdSaCas9-VP64
UseAAV and CRISPRExpressionMammalianPromoterpCMVAvailable SinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV ORANGE Dlg4-HaloTag KI
Plasmid#139656PurposeEndogenous tagging of PSD95: C-terminal (amino acid position: R721)DepositorInsertgRNA and HaloTag donor
UseAAVExpressionMammalianPromoterU6Available SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIO-DSE-mCherry-PSE-MCS
Plasmid#129669PurposeBackbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressedDepositorTypeEmpty backboneUseAAVTagsmCherryPromoterEF1aAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189922PurposeAAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e
UseAAVMutationSaCas9 D10APromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-BoxB
Plasmid#92004PurposeExpresses firefly luciferase with BoxB sequences in 3' UTRDepositorInsertFirefly Luciferase
UseLuciferaseExpressionMammalianAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO_EGFP-GFE3
Plasmid#79871PurposeFor generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.DepositorHas ServiceAAV1InsertGFP-GFE3
UseAAV, Cre/Lox, and Synthetic BiologyTagsGFP and HAExpressionMammalianPromoterEF1aAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash5
Plasmid#74133PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.AX
Plasmid#195218PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)DepositorInsertSynthetic construct isolate AAV-PHP.AX VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI118-SiT-Cas12a-[Ind]
Plasmid#128406PurposeInducible expression of both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVExpressionMammalianAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Lin28
Plasmid#60345PurposeMammalian Expression, LentiviralDepositorAvailable SinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-hCas9-Ce-mRNA
Plasmid#47911PurposeIn vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.DepositorInserthCas9 with C. elegans 5' and 3' UTRs
UseCRISPRPromoterSP6Available SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/td5StayGold(c4)=GianCreg
Plasmid#212022PurposeFor labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].DepositorAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon oG
Plasmid#131778PurposeINTRSECT 2.0DepositorInsertrabies glycoprotein - oG
UseAAV; IntrsectTagsnoneExpressionMammalianPromoterEf1aAvailable SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSGNluc
Plasmid#186705PurposeA high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real timeDepositorInsertsGFP
luciferase
Available SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLC-254
Plasmid#124291PurposeExpression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.DepositorInsertvsfGFP-0
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-EGFPKASH
Plasmid#124882Purposelabelling of neuronal nuclear envelope with EGFPDepositorInsertEGFP
UseAAVTagsKASHPromoterCaMKIIAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
Plasmid#107009PurposeModified Luciferase expression vectorDepositorAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-BFP-sesRNA-tTA2-WPRE-pA
Plasmid#192070PurposeExpression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.DepositorInsertsesRNA for tdTom
UseAAVExpressionMammalianPromoterCAGAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Zscan4 promoter
Plasmid#69249PurposeLuciferase reporter for Dux4 activation of Zscan4 transcriptionDepositorInsertZscan4 (ZSCAN4 Human)
UseLuciferaseMutationThe two single nucleotide mismatches found betwee…PromoterZscan4Available SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Hes1
Plasmid#61534PurposeExpresses Hes1 in mammalian cellsDepositorAvailable SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only