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We narrowed to 18,068 results for: Met;

Showing: 1201 - 1240 of 18068 results

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pgRNA-bacteria_speG_M15

Plasmid

#220488

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Tags

Expression

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_E23

Plasmid

#220489

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Tags

Expression

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speG_genomic

Plasmid

#220473

Purpose

heterologous expression under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA4114

Plasmid

#220474

Purpose

heterologous expression of PA4114 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1472

Plasmid

#220475

Purpose

heterologous expression of PA1472 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1377

Plasmid

#220476

Purpose

heterologous expression of PA1377 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga2p-Flag-CapN-TEVcs-HA

Plasmid

#213530

Purpose

Expresses CapN-caged TEVcs on yeast surface

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

Aga2p-Flag-CapN-TEVcs-HA

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1/10

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-SspB-EGFP-VP16

Plasmid

#213532

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHYX137

Plasmid

#202814

Purpose

Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

ScPCK1

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX138

Plasmid

#202815

Purpose

Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

ScADH2

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX173

Plasmid

#202817

Purpose

Plasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

TEV protease

Use

Tags

Expression

Yeast

Mutation

Promoter

ScPCK1

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW2-MaUPPS-B

Plasmid

#203175

Purpose

Yeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

MA_4402

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1_1-327-dsRNAres_H

Plasmid

#146501

Purpose

Insect Expression of DmDCP1_1-327

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_1-327 (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-dsRNAres_H

Plasmid

#146502

Purpose

Insect Expression of DmDCP1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1 (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)_G

Plasmid

#146402

Purpose

Bacterial Expression of DmDCP1_328-366

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1_327-372_H

Plasmid

#146500

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK028

Plasmid

#180826

Purpose

T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

SwGdmA (SWIT_RS16490 Synthetic, Sphingomonas wittichii RW1)

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK037

Plasmid

#180829

Purpose

T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

CnGdmB (CNE_RS35790 Cupriavidus necator N-1)

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK066

Plasmid

#180830

Purpose

T7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

NaGdmA

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC031

Plasmid

#171152

Purpose

pGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3-BBa_J23117-mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J3-BBa_J23117

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC034

Plasmid

#171153

Purpose

pGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3(106)- BBa_J23111-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J3(106)-BBa_J23111

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC016.109

Plasmid

#171144

Purpose

Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J109 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC008.106

Plasmid

#171141

Purpose

Expression of J106 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J106 scRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTX1-SMN2-exon7-ESE1

Plasmid

#126042

Purpose

In-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

SMN2 exon7 ESE1

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔC

Plasmid

#112653

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔC

Use

Tags

Expression

Yeast

Mutation

R59K, Δ431-493 (deletion of C-terminal amphipathi…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔGRM-ΔC

Plasmid

#112654

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔGRM ΔC

Use

Tags

Expression

Yeast

Mutation

R59K, Δ376-493 (deletion of Glo3 motif & C-te…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 Glo3 R59K S389,398 A

Plasmid

#112656

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K S389,398A

Use

Tags

Expression

Yeast

Mutation

R59K, mutations (S389A, S398A) in Glo3 motif, non…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 GFP-Glo3 ΔGRM-ΔC

Plasmid

#112659

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP-Glo3 ΔGRM ΔC

Use

Tags

sfGFP

Expression

Yeast

Mutation

Δ376-493 (deletion of Glo3 motif & C-terminal…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 GFP-Glo3 S389,398 A

Plasmid

#112660

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP- Glo3 S389,398A

Use

Tags

sfGFP

Expression

Yeast

Mutation

mutations (S389A, S398A) in Glo3 motif, non-phosp…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 Gcs1 R54K L246D

Plasmid

#112648

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K L246D

Use

Tags

Expression

Yeast

Mutation

R54K & L246D (in ALPS motif)

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 Gcs1 R54K AxxA

Plasmid

#112649

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K AxxA

Use

Tags

Expression

Yeast

Mutation

R54K & Alanine substitution of C-terminal δL …

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 Gcs1 Δ3xF AxxA

Plasmid

#112650

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 Δ3xF AxxA

Use

Tags

Expression

Yeast

Mutation

R54K, W304A, W307A, W310A, W349A, F353A

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-ala13

Plasmid

#87174

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proD

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-ala11

Plasmid

#87172

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proA

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-ala12

Plasmid

#87173

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proC

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFPJ1

Plasmid

#83561

Purpose

Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

TEF1 promoter-yEGFP-ERG9 ER signal peptide

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCW022

Plasmid

#83532

Purpose

pheromone mediated expression of UBiC, ARO4, and TKL1

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Insert

pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCW015

Plasmid

#83530

Purpose

UBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoter

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Insert

pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas2B

Plasmid

#82373

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas45

Plasmid

#82390

Purpose

sgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1201 - 1240 of 18068 results

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