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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,350 results for: 11

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Showing: 601 - 620 of 8350 results

  2. N-BLa 1.1

    Plasmid
    #88994
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Publication
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.1 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88994-plasmid-map-sequence-id-177496

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  3. TFORF2205

    Plasmid
    #144004
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    POU2F3 (POU2F3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144004-plasmid-map-sequence-id-391400

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  9. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    71684-plasmid-map-sequence-id-129672

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  10. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Publication
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  11. Flag-M4-AR

    Plasmid
    #171240
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-tagged human androgen receptor (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    171240-plasmid-map-sequence-id-336015

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  15. CIB1-CreC(N1)

    Plasmid
    #75367
    Purpose
    Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase
    Depositor
    Chandra Tucker
    Publication
    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIB1 (CIB1 Mustard Weed)
    Tags
    Cre DNA recombinase, residues 106-343
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    75367-plasmid-map-sequence-id-142710

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  20. C-BLa 1.1

    Plasmid
    #88997
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Publication
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    C-BLa 1.1 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88997-plasmid-map-sequence-id-177504

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Showing: 601 - 620 of 8350 results