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Plasmid#174022PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserttdTomato
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa-KIF5C 559-tdTomato-FKBP
Plasmid#64211PurposeKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tagsDepositorInsertKif5c (Kif5c Rat)
UseTagsFKBP and tdTomatoExpressionMammalianMutationmotor domain aa1-559Promoterchicken Beta ActinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:icre-p2a-tomato
Plasmid#135217PurposeCre coexpressed with tdTomato in macrophagesDepositorInsertmfap4 promoter with iCre and Tomato
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRM18 LTN (Luciferase tdTomato Neo)
Plasmid#174721PurposeExpresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
aph
UseLentiviral and LuciferaseTagsE2A linker and P2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#130909PurposeAAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorHas ServiceAAV8InsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterCAGAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/Ecad-tdTomato
Plasmid#101278PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomatoDepositorInsertE-cadherin
UseLentiviralTagstdtomatoExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-WPRE
Plasmid#51505PurposeCan be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoterDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS423-GPD-wrmScarlet1-10-CYC1 terminator
Plasmid#158584PurposeExpresses wrmScarlet1-10 in S. cerevisiaeDepositorInsertwrmScarlet1-10
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Chronos-tdTomato]
Plasmid#84484PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
15x-QUAS-dTomato-T2A-GCaMP6s
Plasmid#130666PurposeQUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2ADepositorInsert15xQUAS-dTomato-T2A-GCaMP6s
UseAe. aegypti expressionTagsExpressionInsectMutationPromoter15xQUAS-TATAAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
15x-QUAS-dTomato-T2A-TRPV1
Plasmid#140945PurposeQUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2ADepositorInsert15x-QUAS-dTomato-T2A-TRPV1
UseTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1397 DddAtox C
Plasmid#158097Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1397 DddAtox N
Plasmid#158094Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#202610PurposeDouble floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M13-TEV-C-P2A-TdTomato
Plasmid#92389Purposeexpresses Cal-light M13-TEV-C component and TdTomato in mammalian cellsDepositorInsertM13-TEV-C-P2A-TdTomato
UseTagsTdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-tdTomato-P2A-paCaMKII(K42M)
Plasmid#165433PurposeExpresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.DepositorInserttdTomato-P2A-paCaMKII(K42M)
UseTagstdTomatoExpressionMammalianMutationK42M: Kinase dead mutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Puro
Plasmid#135687PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PsbA2-HMGS-HMGR-ATOB-NPTI
Plasmid#73338PurposeConstruct expressing the upper MVA pathway in Synechocystis PCC 6803DepositorInsertHMGS-HMGR-ATOB-NPTI
UseTagsExpressionMutationPromoterpsbA2Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
tetO-Bcl2-IRES-tdtomato(TET005)
Plasmid#117857PurposeThis plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.DepositorInsertBcl2 (Bcl2 Mouse)
UseTta/tetoTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128589PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorHas ServiceAAV8InsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterSynAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only