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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 893 results for: Px330

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Showing: 601 - 620 of 893 results

  1. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135014-plasmid-map-sequence-id-267599

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  3. pTFAP2B.1.0-gDNA

    Plasmid
    #113791
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2B
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TFAP2B (TFAP2B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113791-plasmid-map-sequence-id-230594

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  4. pHEY1.1.0-gDNA

    Plasmid
    #112393
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HEY1 (HEY1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112393-plasmid-map-sequence-id-230239

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  5. AAVS1_Cas9-hGem_gRNA2

    Plasmid
    #217659
    Purpose
    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA2 targeting the human AAVS1 safe harbour locus
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217659-plasmid-map-sequence-id-438226

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  6. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126762-plasmid-map-sequence-id-410965

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  7. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid
    #217662
    Purpose
    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217662-plasmid-map-sequence-id-440854

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  9. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3xFLAG and 126aa domain from HSV-1 UL12 fused to …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  14. pSOX11.1.0-gDNA

    Plasmid
    #176378
    Purpose
    Expresses spCas9 and gRNA sequence targeting SOX11
    Depositor
    Barbara Stranger, Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SOX11 gRNA
    Use
    CRISPR
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176378-plasmid-map-sequence-id-347389

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  15. pBACH2.2.0-gDNA

    Plasmid
    #176375
    Purpose
    Expresses spCas9 and gRNA sequence targeting BACH2
    Depositor
    Barbara Stranger, Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BACH2 gRNA
    Use
    CRISPR
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176375-plasmid-map-sequence-id-347302

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  18. pNFXL1.1.0-gDNA

    Plasmid
    #112451
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NFXL1
    Depositor
    Kevin White
    Insert
    NFXL1 (NFXL1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112451-plasmid-map-sequence-id-230465

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  19. CALID

    Plasmid
    #169708
    Purpose
    Cre-activated light-inducible Dre recombinase
    Depositor
    Dali Li
    Publication
    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CAG
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    169708-plasmid-map-sequence-id-333329

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  20. pSPDEF.1.0-gDNA

    Plasmid
    #113796
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SPDEF
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPDEF (SPDEF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113796-plasmid-map-sequence-id-230613

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Showing: 601 - 620 of 893 results