We narrowed to 13,934 results for: TIM;

1. pH2B-SCFP1-2A-mTq2-NES

   Plasmid

   #60491

   Purpose

   Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm

   Depositor

   Dorus Gadella

   Article

   Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

   Inserts

   H2B-SCFP1
   mTurquoise2-NES
   

   Use

   Tags

   SCFP1 and mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTDpelB-CTwinStrep

   Plasmid

   #45941

   Purpose

   use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   PelB and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. 141_pT3TS_iCre

   Plasmid

   #171793

   Purpose

   Plasmid for optimised iCre RNA synthesis using T3 promoter.

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Insert

   optimised Cre for RNA expression
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T3

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKH-MoHyPer

   Plasmid

   #78267

   Purpose

   Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

   Depositor

   Nicole Donofrio, Jeffrey Caplan

   Article

   Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

   Insert

   MoHyper
   

   Use

   Express in magnaporthe

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Rp27

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pLIC.B3.TM0851

   Plasmid

   #11530

   Depositor

   Sung-Hou Kim

   Article

   Liu et al J Mol Biol. 2005 Jul 29. 350(5):987-96.

   Insert

   heat-inducible transcriptional repressor (TM0851 T. maritima)
   

   Use

   Tags

   HisTag, TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-pfv-Sapphire

   Plasmid

   #231957

   Purpose

   The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)

   Depositor

   Michael Shtutman

   Article

   Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.

   Insert

   genetically encoded multimeric nanoparticles
   

   Use

   Lentiviral; Doxocycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUB_SM-FLuc0-GFPOPT

   Plasmid

   #210594

   Purpose

   Expresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFP

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-nonoptimal FLuc-Optimal GFP
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB_SM-Fluc100-GFPOPT

   Plasmid

   #210596

   Purpose

   Expresses SM(FLAG)-optimal Firefly luciferase-optimal GFP

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-optimal FLuc-Optimal GFP
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRS-dCas9

   Plasmid

   #220193

   Purpose

   mini-Tn7 dCas9 expression vector

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   promoterless

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ1651-sgTelomere(F+E)

    Plasmid

    #51024

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

    Depositor

    Stanley Qi, Bo Huang

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    Optimized sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRS-XylS-dCas9

    Plasmid

    #220196

    Purpose

    mini-Tn7 dCas9 expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    XylS/pM

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTD-NTwinStrep_Sm

    Plasmid

    #45937

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD_Pinx-1::ChR2(H134R)::mCherry

    Plasmid

    #117420

    Purpose

    For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    channel rhodopsin-2 with H134R mutation
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    inx-1

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRS-LacI-dCas9

    Plasmid

    #220194

    Purpose

    mini-Tn7 dCas9 expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    LacI/pLac

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid

    #60492

    Purpose

    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm

    Depositor

    Dorus Gadella

    Article

    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

    Inserts

    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    

    Use

    Tags

    SGFP2 and SGFP2(T65G)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD_Psra-6::ChR2(H134R)::mCherry

    Plasmid

    #117419

    Purpose

    For selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoter

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    channel rhodopsin-2 with H134R mutation
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    sra-6

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUGW-RapACR-EYFP

    Plasmid

    #111075

    Purpose

    Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNB813

    Plasmid

    #60161

    Purpose

    Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNB784

    Plasmid

    #60163

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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