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Plasmid#166992PurposeTests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
1026L=U6-3-gRNA#1[Pol-γ35]
Plasmid#159674PurposePlasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.DepositorInsertU6-3-gRNA#1[Pol-γ35]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
1026M=U6-3-gRNA#2[Pol-γ35]
Plasmid#159675PurposePlasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.DepositorInsertU6-3-gRNA#2[Pol-γ35]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v4)-PGK-Puro-BFP
Plasmid#117143PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v4 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v5)-PGK-Puro-BFP
Plasmid#117144PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v5 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP
Plasmid#117140PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v1 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP
Plasmid#117141PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v2 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120394PurposepAAV plasmid expressing Diras2 shRNA2 under the U6 promoter and mCherry.3XFLAG under the hSyn promoterDepositorInsertDiras2 shRNA (Diras2 Mouse)
UseAAV and RNAiTagsmCherry.3XFLAGExpressionMutationPromoterU6Available sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP
Plasmid#114730PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M7
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP
Plasmid#114728PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M5
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L1-L4
Plasmid#62136PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA R4-R3
Plasmid#62137PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L5-L2
Plasmid#62139PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) U6-SYT7 sgRNA 777 HaloTag
Plasmid#179724PurposeU6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITIDepositorInsertsgRNA and HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Multiple Lentiviral Expression (MuLE) system
Plasmid Kit#1000000060PurposeMultiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.DepositorAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP
Plasmid#163030PurposeExpression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianMutationPromoterAlb-AATAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9
Plasmid#163025PurposeExpression of CRISPRi with gRNA against mouse Pcsk9DepositorInsertMammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianMutationPromoterAlb-AATAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR
Plasmid#207878PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR
Plasmid#207880PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5
Plasmid#124362PurposeOverexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)DepositorInsertTornado-F30-Broccoli-aptNFkB#5
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only