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pET-Cas9-6xHis

Plasmid

#62374

Purpose

Expression of Cas9-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9_6xHis

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

Encodes anti-HA scFv (anti-HA frankenbody) fused with mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pcDNA3.2/v5-DEST hGlut1

Plasmid

#18085

Depositor

Wolf Frommer

Publication

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Glut1 (SLC2A1 Human)

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pK-myc-Par3b

Plasmid

#19388

Depositor

Ian Macara

Publication

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Partitioning-defective protein 3b (PARD3 Human)

Tags

Myc

Expression

Mammalian

Mutation

Full length, isoform b.

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLVX-UbC-rtTA-Ngn2:2A:EGFP

Plasmid

#127288

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

Depositor

Fred Gage

Publication

Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

Insert

Ngn2:2A:Ascl1 (NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and EGFP linked via 2A

Expression

Mammalian

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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GFP-Ub KO.G76V

Plasmid

#11932

Depositor

Nico Dantuma

Publication

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ubiquitin KO.G76V (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Conjugation deficient mutant. All 7 lysines of ub…

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MSCV PRDM16

Plasmid

#15504

Depositor

Bruce Spiegelman

Publication

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (isoform 4) (Prdm16 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Flag-HA-USP22

Plasmid

#22575

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP22 (USP22 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF5B-FRT-GFP-αTAT1

Plasmid

#27099

Depositor

Maxence Nachury

Publication

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

α-Tubulin K40 acetyltransferase1 (ATAT1 Human)

Tags

GFP, S-tag, and TEV site

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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E[beta]C

Plasmid

#24312

Depositor

Roel Nusse

Publication

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)

Use

Lentiviral

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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LentiV_Cas9_puro

Plasmid

#108100

Purpose

Lentiviral expression plasmid of spCas9 with puromycin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

59 Articles

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pLenti6.2-ccdB-3xFLAG-V5

Plasmid

#87071

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Publication

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pB-GLUT4-7myc-GFP

Plasmid

#52872

Purpose

detection and measurement of GLUT4 translocation

Depositor

Jonathan Bogan

Publication

Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

Insert

GLUT4 (Slc2a4 Rat, with human c-terminus)

Use

Retroviral

Tags

7x-myc (internal) and GFP

Expression

Mammalian

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

Plasmid

#101139

Purpose

Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Publication

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDONR207 SARS-CoV-2 E

Plasmid

#141273

Purpose

Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

E (E SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

Plasmid

#153005

Purpose

Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Publication

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (C) (G79)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pLEX-MCS-ASC-GFP

Plasmid

#73957

Purpose

Express human ASC with a C-terminal EGFP tag in mammalian cells

Depositor

Christian Stehlik

Publication

de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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