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pOZ-N-FH PSMD14

Plasmid

#27500

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

PSMD14 (PSMD14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

M1V

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only
M10.2 Southern Blot 5'-external probe

Plasmid

#15861

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

M10.2

Expression

Bacterial

Available Since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pET19b-MED29

Plasmid

#15415

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED29 (MED29 Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pL6-H2B-iRFP720-bla

Plasmid

#189991

Purpose

Lentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistance

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Human histone H2B

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pL6-H2B-iRFP720-puro

Plasmid

#189992

Purpose

Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Human histone H2B

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-Flag

Plasmid

#210349

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot7_AF

Plasmid

#148741

Purpose

Bacterial Expression of HsNot7

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot7 (CNOT7 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRRP6_Z

Plasmid

#148131

Purpose

Mammalian Expression of HsRRP6

Depositor

Elisa Izaurralde

Insert

HsRRP6 (EXOSC10 Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence of is…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJH643

Plasmid

#172168

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam

Expression

Bacterial

Mutation

sfGFP(N39*, N135*, Y151*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BTB)

Plasmid

#169721

Purpose

Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS

Plasmid

#162610

Purpose

Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

Contains aa1-155 of mVenus

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K9-2xCSTREP

Plasmid

#136160

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K9

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Gal4-entry

Plasmid

#128014

Purpose

Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

Gal4-DBD-3xFlag

Use

Gal4 entry vector

Tags

Gal4-DBD-3xFlag

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MUS2-1246-vpr

Plasmid

#115841

Purpose

Encodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMUS21246 Vpr (EF070329)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSR26-2

Plasmid

#104335

Purpose

Expresses ompR from a TetR reuglated promoter under inducible ATC control.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

OmpR (ompR Escherichia coli str. K-12 substr. MG1655)

Expression

Bacterial

Promoter

PLTetO1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRC-1/1-IS2/2

Plasmid

#91251

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS2/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (1-200)

Plasmid

#86750

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 1-200

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHcPro (GB0067)

Plasmid

#68196

Purpose

Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

TEV HcPro coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNTAQ1

Plasmid

#65486

Purpose

Mouse Ntaq1-EGFP reporter

Depositor

Alexander Varshavsky

Article

Wang et al Mol Cell. 2009 Jun 26;34(6):686-95. doi:

Insert

Ntaq1 (Wdyhv1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only