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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,423 results for: tes

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Showing: 6201 - 6220 of 17423 results

  4. pcDNA3-HA-ERK5kin

    Plasmid
    #65245
    Purpose
    Expression of HA tagged ERK5 kinase domain in mammalian cells
    Depositor
    Axel Ullrich
    Publication
    Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERK5kin (MAPK7 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    deleted AA 410-817
    Promoter
    CMV
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. p404MET25

    Plasmid
    #17420
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    MET25 promoter is between SacI and XbaI restricti…
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17420-plasmid-map-sequence-id-8595

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  8. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid
    #67277
    Purpose
    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
    Depositor
    Jannik Elverløv-Jakobsen
    Publication
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Inserts
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    Tags
    mTQ2 blue fluorescent and red flourescent gene ka…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67277-plasmid-map-sequence-id-121189

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  10. pKb-TnpB2

    Plasmid
    #215334
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Publication
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Tags
    Not applicable
    Expression
    Plant
    Promoter
    Rice ubiquitin promoter for TnpB and maize ubiqui…
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215334-plasmid-map-sequence-id-447325

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  11. pCDF-Mm2

    Plasmid
    #197100
    Purpose
    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati
    Depositor
    Kensaku Sakamoto
    Publication
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    PylRS variant, Pyl tRNA, kanamycin resistance gene
    Expression
    Bacterial
    Mutation
    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197100-plasmid-map-sequence-id-393189

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  12. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Publication
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  13. pcDNA5-FRT/TO-eGFP-SHLD3

    Plasmid
    #114125
    Purpose
    Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells
    Depositor
    Daniel Durocher
    Publication
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SHLD3 (SHLD3 Human)
    Use
    Genomic integration, tetracycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV/TetO2
    Available since
    Aug. 16, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114125-plasmid-map-sequence-id-218642

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  15. pmIL-6 mut C/EBP

    Plasmid
    #61291
    Purpose
    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site
    Depositor
    Gail Bishop
    Publication
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to…
    Promoter
    IL-6 promoter with mutant C/EBP binding site
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  16. CCL5-bio-His

    Plasmid
    #52027
    Purpose
    Expresses full-length C-C motif chemokine 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CCL5 (CCL5 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52027-plasmid-map-sequence-id-80101

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Showing: 6201 - 6220 of 17423 results