We narrowed to 10,238 results for: ada

1. pKK-TEV-mClover3

   Plasmid

   #105795

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mClover3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Puro-sTagRFP-TUBA1B

   Plasmid

   #207769

   Purpose

   Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pD40-His/V5-c-MycS62A

   Plasmid

   #45599

   Depositor

   Rosalie Sears

   Article

   Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

   Insert

   c-Myc S62A (Myc Mouse)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   S62A

   Promoter

   CMV

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

4. AAV-GfABC1D-SaCas9-WPRE3-pA

   Plasmid

   #203540

   Purpose

   SaCas9 expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   SaCas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EFS-DIO-GRAB_NTS1.0

   Plasmid

   #208671

   Purpose

   Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-EGFP-CAAX

   Plasmid

   #208684

   Purpose

   Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   membrane-localized enhanced green fluorescent protein EGFP-CAAX
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcsII-pb4-flag

   Plasmid

   #86768

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   proteasome beta subunit 4 (PSMB4 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1_MeCP2(R306C)

   Plasmid

   #110188

   Purpose

   Expression of mouse MeCP2 (R306C) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Arg 306 Cys

   Promoter

   CMV

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-TEV-mVenus

   Plasmid

   #105791

   Purpose

   Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mVenus

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

    Plasmid

    #163572

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC_hfnCas12a_NR1_NR2

    Plasmid

    #176252

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-NSD3-short

    Plasmid

    #72552

    Purpose

    expresses 3*FLAG tagged human NSD3-short

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA-gp78-FLAG RING mutant C356G H361A

    Plasmid

    #61751

    Purpose

    express C-terminally FLAG tagged human gp78

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    gp78 (AMFR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C356G H361A; P179L, F262L, Y315C, and D346G (ple…

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCold I-Hero11

    Plasmid

    #187928

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero11

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero11 (C19orf53 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2R3a-3xVenusYFP-OcsT

    Plasmid

    #71271

    Purpose

    Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    3 times VenusYFP
    

    Use

    Gateway

    Tags

    octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-mCherry-FKBP-GAP(ARHGAP29)

    Plasmid

    #222632

    Purpose

    Rho inactivation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
    

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

    Plasmid

    #101062

    Purpose

    AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    CaMPARI2-H396K
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-GRAB_SST1.0

    Plasmid

    #208666

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-FCHO1

    Plasmid

    #196910

    Purpose

    visualization of clathrin-coaed vesicle formation in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

    Insert

    FCHO1 (FCHO1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1_MeCP2(deltaNC)

    Plasmid

    #110190

    Purpose

    Expression of mouse MeCP2 (deltaNC) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta N and C termini

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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