We narrowed to 59,563 results for: ran

1. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

   Plasmid

   #202719

   Purpose

   Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Myr-Pink Flamindo
   EGFP-bPAC-CAAX
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p CIneo-RL-Let7-3xBulgeB

   Plasmid

   #115368

   Purpose

   Expression vector to produce humanized Renilla luciferase with three partially complementary binding sites for human let-7 miRNA in the 3'UTR

   Depositor

   Witold Filipowicz, Ramesh Pillai

   Article

   Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR221-CAG.Pro

   Plasmid

   #79490

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   CAG Promoter
   

   Use

   Multisite gateway entry clone

   Promoter

   CAG Promoter

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. plc242-GFP-Nprl2

   Plasmid

   #87051

   Purpose

   mammalian expression of Nprl2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   Nprl2 (NPRL2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAdv/TERT

   Plasmid

   #26744

   Depositor

   Bingliang Fang

   Article

   Gu et al Cancer Res. 2000 Oct 1. 60(19):5359-64.

   Insert

   human TERT promoter (TERT Human)
   

   Expression

   Mammalian

   Available Since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPRIME-dsRed-shvgat

   Plasmid

   #71385

   Purpose

   Expresses dsRed and shRNA against vgat under the control of the CMV promoter in eukaryotic cells

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   CMV promoter-dsRed-mir30-shvgat
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   CMV enhancer/promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Plas-gRNA-Z3EV

   Plasmid

   #157659

   Purpose

   GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   GFP-gRNA NatMX Z3EV
   

   Use

   Insert storage (replicative in e. coli)

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. BSP188

   Plasmid

   #110917

   Purpose

   For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

   Depositor

   Alexander Mendenhall

   Article

   Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

   Insert

   unc54 Terminator
   

   Use

   Synthetic Biology; Yeast homologous recombination…

   Expression

   Worm

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEL1203

   Plasmid

   #196024

   Purpose

   Expression/purification of 6xHis-TEV-MlaC

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaC
   

   Expression

   Bacterial

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. BcLOV-mCh-EGFR

    Plasmid

    #208274

    Purpose

    Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    BcLOV-mCherry-EGFR
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    pCMV

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AY42_pS.Donor.R5

    Plasmid

    #100291

    Purpose

    CCR5-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRuby2-MyosinIIA-C-18

    Plasmid

    #55906

    Purpose

    Localization: Cytoskeleton, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    MyosinIIA
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. p CIneo-RL-Let7-perf

    Plasmid

    #115367

    Purpose

    Expression vector to produce humanized Renilla luciferase with a single perfectly complementary binding site for human let-7 miRNA in the 3'UTR

    Depositor

    Witold Filipowicz, Ramesh Pillai

    Article

    Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK506

    Plasmid

    #155382

    Purpose

    dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDrop

    Plasmid

    #184872

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAWH

    Plasmid

    #133894

    Purpose

    Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBKWH

    Plasmid

    #133898

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pColE1_sgRNA_M1S_AmpR

    Plasmid

    #119153

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pColE1_sgRNA_M1NS_AmpR

    Plasmid

    #119155

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTetQCas-BsaI

    Plasmid

    #170629

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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