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pLS Series: Minimal Shuttle Vectors for Saccharomyces cerevisiae

Plasmid Kit

#1000000267

Purpose

Minimal shuttle vectors of reduced size for Escherichia coli and Saccharomyces cerevisiae.

Depositor

Sahand Jamal Rahi

Application

Gene Expression and Labeling

Vector Type

Bacterial Expression, Yeast Expression

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only
pDZ2087

Plasmid

#92414

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

Insert

TEV protease

Tags

MBP (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

tac

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET28 MBP_PrA/G-H6

Plasmid

#176077

Purpose

Express His6 tagged MBP_PrA/G in Escherichia coli

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

MBP PrA/G-H6

Tags

His tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETA_crGE

Plasmid

#172927

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pIMAY

Plasmid

#68939

Purpose

E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

Depositor

Tim Foster

Article

Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

Type

Empty backbone

Use

Staphylococcal allelic exchange vector

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET303_sumocodo_GFP_codoopti

Plasmid

#210393

Purpose

Express SUMO-GFP in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

GFP

Tags

SUMO-tag

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEcoFus

Plasmid

#187833

Purpose

1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpression

Depositor

Jörg Vogel

Article

Ponath et al Nat Microbiol. 2021 Aug;6(8):1007-1020.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGS-21a his6-GST-WTPhi29DNAP

Plasmid

#166863

Purpose

To produce a dual affinity-tagged Phi29 DNA polymerase

Depositor

Paul Blainey, Stephen Quake

Article

Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

Insert

Phi29 Polymerase codon-optimized for overexpression in E. coli

Tags

GST and his6

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTatABCmNeon

Plasmid

#178465

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatB, TatCmNeon

Tags

mNeonGreen fused to TatC

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(0)

Plasmid

#199166

Purpose

used to express a neutral GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(0)

Tags

MGHHHHHHGGA

Mutation

E16R, I138R, D207R, N222K, L246R

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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Salmonella MTAN

Plasmid

#64041

Purpose

Expression of S. enterica MTAN in E. coli

Depositor

Vern Schramm

Article

Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

Insert

Methylthioadenosine nucleosidase

Tags

Histidine tag

Expression

Bacterial

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Tags

MGHHHHHHGGA

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJExpress401-T4 Lysozyme C54T C97A (WT*)

Plasmid

#224353

Purpose

Expresses T4 lysozyme in E. coli

Depositor

Terry Watt

Article

Toro et al MethodsX. 2015 May 18;2:256-62. doi: 10.

Insert

T4 Lysozyme

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

C45T, C94A

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pG1AK-sfGFP

Plasmid

#71739

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

sfGFP

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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Pet28a-His-tag-BS2

Plasmid

#220347

Purpose

E. coli expression vector to purify BS2

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

His tag

Expression

Bacterial

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSK+KanaRpsL

Plasmid

#20871

Depositor

Jiyue Zhu

Article

Wang et al Mol Biotechnol. 2009 May;42(1):110-6. do

Insert

KanamycinResistance-RpsL

Expression

Bacterial

Mutation

none

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCAGTetRnls

Plasmid

#26599

Depositor

Peter Andrews

Article

Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

Insert

Tetracycline repressor (TetR E.Coli)

Tags

nls

Expression

Mammalian

Available Since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pSG86-J23151-LasR

Plasmid

#102456

Purpose

Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

LasR (lasR )

Expression

Bacterial

Promoter

J23151

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-chmI

Plasmid

#221341

Purpose

Express ChmI in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmI

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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