We narrowed to 12,973 results for: NUC

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pTY152 EGFP-T2A-ORF6 SARS-CoV-1

Plasmid

#204974

Purpose

Co-express EGFP and SARS-CoV-1 ORF6

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-1)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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POT4-RFP

Plasmid

#65320

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAP118

Plasmid

#105495

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

HupA-SmBiT/HupA-LgBiT

Use

Atc-dependent expression in c. difficile

Promoter

Ptet

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1-del1174-1649_AH

Plasmid

#148904

Purpose

Mammalian Expression of HsXRN1-del1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1-del1174-1649 (XRN1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-XPLN

Plasmid

#73375

Purpose

GST-tagged expression of XPLN (ARHGEF3) in bacteria

Depositor

Jie Chen, Krister Wennerberg

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Tags

GST

Expression

Bacterial

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
BirA*-HA-NES

Plasmid

#214473

Purpose

Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

Depositor

Natalie Ahn

Article

Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

Insert

BirA(R118G)

Use

Lentiviral; Gateway

Tags

HA tag and NES (Nuclear Exclusion Sequence)

Expression

Mammalian

Mutation

R118G

Promoter

EF-1a

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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KN206: pMVP/eCFP-DEST

Plasmid

#121854

Purpose

pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1p shDna2

Plasmid

#31951

Depositor

Sheila Stewart

Article

Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

Insert

shRNA against hDna2 (DNA2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
p2xFlag CMV2-YAP2-deltaC

Plasmid

#21123

Depositor

Marius Sudol

Article

Tsutomu Oka & Marius et al

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

5 Carboxyterminal amino acids -FLTWL are deleted

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pBABEhygro-Flag-ZO-2

Plasmid

#27473

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pTZ tRNA_gRNA empty scaffold

Plasmid

#188450

Purpose

Expression of gRNAs using a human tRNA promoter

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

tRNA glutamine

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-HA-dSpCas9

Plasmid

#92112

Purpose

Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9

Use

CRISPR

Tags

HA tag and NLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_641-1574_AH

Plasmid

#148920

Purpose

Insect Expression of DmGIGYF_641-1574

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_641-1574 (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_1-640_AH

Plasmid

#148919

Purpose

Insect Expression of DmGIGYF_1-640

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_1-640 (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pY006 (pcDNA3.1-hBpCpf1)

Plasmid

#69978

Purpose

Expresses humanized BpCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hBpCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
JH401: pMVP/Ad/RGD-PK-DEST

Plasmid

#121846

Purpose

pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pQsupR-Mi2b

Plasmid

#15379

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

short hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)

Use

RNAi and Retroviral

Available Since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
eGFP Y93H GC editing reporter

Plasmid

#198884

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGG)

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCL1316

Plasmid

#217958

Purpose

Expression of human H4 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H4 (H4C1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-Flag-ZO-2

Plasmid

#27420

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only