We narrowed to 11,733 results for: aga

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ADK gRNA (BRDN0001146410)

Plasmid

#75743

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADK (ADK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5b HA Irx5 deltaIrot

Plasmid

#24995

Depositor

Benoit Bruneau

Article

Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

Insert

Irx5 (Irx5 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of Iro box (aa 314-327), 19 aa upstream …

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5b HA Irx5 deltaC

Plasmid

#24996

Depositor

Benoit Bruneau

Article

Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

Insert

Irx5 (Irx5 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletes everything after Hox Domain (aa 112-177).…

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pLENTICRISPR-LUC7L3-sgRNA1

Plasmid

#165937

Purpose

CRISPR-mediated gene perturbation (knock-out) of LUC7L3

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L3 (LUC7L3 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158653

Purpose

making transgenics expressing a UAS driven mCherry and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP

Plasmid

#158654

Purpose

making transgenics expressing a QUAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRP.ABCA4 c.5461-10T

Plasmid

#226553

Purpose

Minigene encoding the ABCA4 c.5461-10T mutation

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

ABCA4 (ABCA4 Human)

Expression

Mammalian

Mutation

c.5461-10T intronic mutation

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDA in pMAX

Plasmid

#120402

Purpose

enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Article

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

EDA-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.8D2Q.Tau2N4R

Plasmid

#226398

Purpose

Expresses his-tagged, 3C-cleavable 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

His.3C

Expression

Bacterial

Mutation

8D2Q.Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158652

Purpose

making transgenics expressing a UAS driven eGFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-GCN5

Plasmid

#65386

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

GCN5 (KAT2A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgRNA-BFP-Puro

Plasmid

#229014

Purpose

Perturb Seq lentiviral sgRNA vector

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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Nluc CD63

Plasmid

#242528

Purpose

This plasmid can be used to quantify CD63 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.

Depositor

Clotilde Thery

Article

Grisard et al J Extracell Vesicles. 2022 Jul;11(7):e12

Insert

CD63 (CD63 Human)

Use

Luciferase

Tags

Nanoluciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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GFP-BRD2

Plasmid

#65376

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD2 (BRD2 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCX-HO1-2A-EGFP

Plasmid

#74672

Purpose

Mammalian expression of HO1 and EGFP

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Insert

HO1 (HMOX1 Human)

Tags

2A-EGFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-BAZ1B

Plasmid

#65372

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BAZ1B (BAZ1B Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A53T

Plasmid

#181738

Purpose

Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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TERT - WT

Plasmid

#213827

Purpose

Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

human TERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-rsChRmine-oScarlet-Kv2.1-WPRE

Plasmid

#183529

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

I146M/G174S

Promoter

Ef1a

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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