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763 pME mApple

Plasmid

#195960

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHJ8: CadC-VHH-Control

Plasmid

#108243

Purpose

membrane bound transcription activator CadC fusing with anti-RNase A antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Membrane bound transcription activator CadC fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTnsABC

Plasmid

#160031

Purpose

Expresses V. cholerae TnsABC

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Mutation

no

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pgRNAtet-lvaA

Plasmid

#106397

Purpose

Guide RNA plasmid targeting lvaA on BBR1-UP origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sgRNA towards lvaA in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSL4393 (pClpX_Eco)

Plasmid

#200907

Purpose

Expresses human codon-optimized E. coli ClpX from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

EcoClpX

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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CBla_2.0_EmrE_TMD4_3P

Plasmid

#207848

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

C-BLa 2.0 fusion protein

Tags

Flag (DYKDHDG)

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJBL7045

Plasmid

#167216

Purpose

overexpression of CueR in E. coli for cell-free copper sensing

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

CueR

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7062

Plasmid

#167231

Purpose

cell-free cascaded copper sensor

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pCue-AKSIRV

Use

Synthetic Biology

Tags

6X His

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV

Plasmid

#235534

Purpose

To express GFP

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - NFDD

Plasmid

#89624

Purpose

Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD/FAD dependent dehydrogenase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL7056

Plasmid

#167225

Purpose

orthogonal T7 RNAP sfGFP reporter

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-sfGFP

Use

Synthetic Biology

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7044

Plasmid

#167215

Purpose

overexpression of PbrR in E. coli for cell-free lead sensing

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

PbrR

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7061

Plasmid

#167230

Purpose

cell-free cascaded lead sensor

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pPbr-AKSIRV

Use

Synthetic Biology

Tags

6X His

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAS006

Plasmid

#211506

Purpose

AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Type

Empty backbone

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGFAP-TetOn3G

Plasmid

#133728

Purpose

Plasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G

Use

AAV

Expression

Mammalian

Promoter

pGFAP

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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mRuby2-Lifeact-7

Plasmid

#54674

Purpose

Localization: Actin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Lifeact-mRuby2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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PBCAG-EGFR

Plasmid

#40974

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDA019

Plasmid

#131452

Purpose

control expression vector

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Type

Empty backbone

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP

Plasmid

#121907

Purpose

Expresses BirA and eCPX-AP

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
Enhanced Circularly Permuted Outer Membrane Protein X

Tags

6xHis

Expression

Bacterial

Promoter

T7 and pBAD promoter

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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