We narrowed to 10,305 results for: otos
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Plasmid#107476PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K37Q approximately wildtype activityDepositorAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCEP4-SERTL406I-myc
Plasmid#107487PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L406I with approximately wildtype activityDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTE392M-myc
Plasmid#107486PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E392M with approximately wildtype activityDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTT233F-myc
Plasmid#107481PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T233F with approximately wildtype activityDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI576V-myc
Plasmid#107473PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transportDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTL550V-myc
Plasmid#107472PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L550V approximately wildtype transportDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB434
Plasmid#68699PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsGlucocorticoid receptor (GR)ExpressionPlantPromoterMpHSP17.8A1 promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB433
Plasmid#68698PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterMpHSP17.8A1 promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB410
Plasmid#68675PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTags3xFLAGExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB413
Plasmid#68678PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsGlucocorticoid receptor (GR)ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB233
Plasmid#68624PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterMpHSP17.8A1 promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB210
Plasmid#68601PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTags3xFLAGExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
dEos-Vinculin-14
Plasmid#57568PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdEos-Supervillin-45
Plasmid#57671PurposeLocalization: Membrane/Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertSupervillin
TagstdEosExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK5_tAKARδ
Plasmid#119916PurposeMammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARδ
ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARε
Plasmid#119917PurposeMammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARε
ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCI SMN1
Plasmid#72286PurposeSMN1 exon 7 splicing cassetteDepositorAvailable SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
ePPEplus
Plasmid#205241PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
TagsStrep(II)-tagExpressionBacterialPromoterT7 PromotorAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L-R279E
Plasmid#182282PurposeMammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.DepositorInsertcAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
ExpressionBacterialMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257AAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L
Plasmid#182283PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L-R279E
Plasmid#182286PurposeMammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21
Plasmid#105522PurposeT7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNADepositorAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGE011
Plasmid#71537PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-c-Jun
Plasmid#40348DepositorAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBK-CMV-hNHERF2
Plasmid#47801PurposeExpresses human NHERF2 in mammalian cellsDepositorAvailable SinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only