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pFA6a-6xGLY-VSV-G-hphMX4

Plasmid

#20773

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-QFGal4-SV40pA

Plasmid

#155126

Purpose

In vitro transcription of QFGal4 mRNA

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Expression in zebrafish

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-ySet2

Plasmid

#51698

Purpose

Expresses full length yeast Set 2 gene in a mamalian expression vector

Depositor

Tom Misteli

Article

Luco et al Science. 2010 Feb 19;327(5968):996-1000.

Insert

yeast Set2 (SET2 Budding Yeast)

Tags

C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only
pLENTI_RPS2

Plasmid

#127127

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2031

Plasmid

#49134

Purpose

AAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple305-iCre

Use

AAV

Expression

Mammalian

Promoter

OLIG1

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-UGI-nCas9(H840A)-GSVG

Plasmid

#187411

Purpose

DddA GSVG variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-TKFC in pLJM60

Plasmid

#154206

Purpose

Lentiviral vector for stable expression of TKFC

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TKFC (DAK Human)

Use

Lentiviral

Tags

N terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS34

Plasmid

#99327

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS34 (PIK3C3 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only
pISceI Dusp6:memGFP

Plasmid

#32668

Purpose

Expresses membrane localized EGFP under the Dusp6 promoter

Depositor

Michael Tsang

Article

Wang et al Development. 2011 Jan;138(1):45-54. Epub

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-BRSK1

Plasmid

#23597

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRSK1 (BRSK1 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pG6(5'Pro) (P#1477)

Plasmid

#14659

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

UAS repeats

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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HHV7 U69

Plasmid

#26693

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

U69 (U69 Human Herpes Virus 7)

Tags

HA

Expression

Mammalian

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TK1

Plasmid

#23736

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TK1 (TK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NAGK

Plasmid

#23785

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NAGK (NAGK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
XE150 DN p53-pCS2P+

Plasmid

#17033

Depositor

Randall Moon

Insert

dominant negative p53 (TP53 Human)

Use

Xenopus expression

Mutation

Dominant negative

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pPM1001

Plasmid

#167548

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Inaba serotype

Use

Cosmid

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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GRK2-CAAX

Plasmid

#166224

Purpose

GRK2-CAAX mammalian expression plasmid

Depositor

Robert Lefkowitz

Article

Inglese et al Nature. 1992 Sep 10;359(6391):147-50. do

Insert

bovine GRK2-CAAX (GRK2 Bovine)

Tags

no

Expression

Mammalian

Mutation

adding CAAX sequence at C terminal

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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