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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,836 results for: construct

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Showing: 6341 - 6360 of 8836 results

  2. pJB02

    Plasmid
    #111959
    Purpose
    Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    T7
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111959-plasmid-map-sequence-id-219913

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  5. p-mCherry-C1-GtACR2

    Plasmid
    #85463
    Purpose
    Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)
    Depositor
    Peter Hegemann
    Article
    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.
    Insert
    Synthetic construct ACR2 gene
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV (+enhancer)
    Available Since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85463-plasmid-map-sequence-id-170777

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  6. p-mCherry-C1-GtACR1

    Plasmid
    #85464
    Purpose
    Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)
    Depositor
    Peter Hegemann
    Article
    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.
    Insert
    Synthetic construct ACR1 gene
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV (+enhancer)
    Available Since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85464-plasmid-map-sequence-id-170772

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  12. pSLC-255

    Plasmid
    #124292
    Purpose
    Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Article
    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available Since
    Sept. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    124292-plasmid-map-sequence-id-243084

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  16. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid
    #227245
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1α and pTRE3G
    Available Since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227245-plasmid-map-sequence-id-450568

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  18. pV92.2

    Plasmid
    #78409
    Purpose
    pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct
    Depositor
    Andrew Fire
    Article
    Silas et al Science. 2016 Feb 26;351(6276):aad4234.
    Inserts
    RT-Cas1
    Cas2
    Available Since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    78409-plasmid-map-sequence-id-146291

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Showing: 6341 - 6360 of 8836 results