We narrowed to 10,386 results for: Coli

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pTAMAHISTEV_BauA

Plasmid

#128952

Purpose

expresses BauA in E.coli

Depositor

James Naismith

Article

Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

Insert

ferric acinetobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-66)

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Wild-type CoV-2 ORF8

Plasmid

#159933

Purpose

Protein overexpression in E.coli

Depositor

James Hurley

Article

Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

CoV-2 ORF8 18-121 (ORF8 CoV-2)

Tags

6x Histidine, TEV cleavable

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pACYC-T7-Nla-repeat-BbsI

Plasmid

#180215

Purpose

For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX3x-hRanBP2-(IR1+M)

Plasmid

#53140

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2631-2711)

Promoter

tac

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b/BOA

Plasmid

#82041

Purpose

For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

Depositor

Angela Gronenborn

Article

Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

Insert

Burkholderia oklahomensis agglutinin

Tags

6x His

Expression

Bacterial

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET30 GLTP

Plasmid

#170733

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCOLA kiwi sensor sfGFP

Plasmid

#107368

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

kiwi-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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mCas13 Twin-SUMO

Plasmid

#199753

Purpose

Expresses codon optimized miniature mCas13 for protein expression in E.coli

Depositor

Magdy Mahfouz

Article

Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

Insert

mCas13

Tags

SUMO

Expression

Bacterial

Promoter

LacI

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ915v2+Nterm Spe1 +Cterm Age1 site

Plasmid

#88914

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 77-465

Plasmid

#45970

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 77-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pST-KNarK2

Plasmid

#44565

Purpose

for ectopic hypoxia inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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His-Sin3a-115-212(pL01)

Plasmid

#124386

Purpose

E.Coli produced SIN3A PAH1 purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

His-Sin3a-115-212 (SIN3A )

Tags

His

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBXPHM3-PCFT_NB

Plasmid

#165415

Purpose

Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT_NB (SLC46A1 Llama, Chicken)

Tags

His10-MBP-3C protease site

Expression

Bacterial

Promoter

arabinose

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin W403A

Plasmid

#45974

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEBB-HA-hILP del RING

Plasmid

#25675

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

Encodes aa 1-449 only. aa 450-497 have been dele…

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-parkin C431S

Plasmid

#45976

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin C431S (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET151-mUCH37 (ISF2)

Plasmid

#61928

Purpose

Expression of mUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

UCH37 (M.musculus) (Uchl5 Mouse)

Tags

6x HIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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V32M,L84M CoV-2 ORF8

Plasmid

#159934

Purpose

Protein overexpression in E.coli

Depositor

James Hurley

Article

Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

CoV-2 ORF8 18-121 (ORF8 CoV-2)

Tags

6x Histidine, TEV cleavable

Expression

Bacterial

Mutation

V32M, L84M

Promoter

T7

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin F463A

Plasmid

#45975

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin F463A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET-GST-05230

Plasmid

#67874

Purpose

E.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.

Depositor

Armin Djamei

Article

Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

Insert

shy1

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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4E2H.210_LUMABS

Plasmid

#155209

Purpose

Expresses 4E2H.210_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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A102-GAP-BoCCS-GRM

Plasmid

#123286

Purpose

Expression of Glo3 ΔAmpH (1-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cat_TU

Plasmid

#127051

Purpose

Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Cat_TU

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin F146A

Plasmid

#45973

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin F146A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pNP1 AND gate sfGFP

Plasmid

#107361

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

AND gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNH002

Plasmid

#42145

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP TAG::[(G4S)3]::mTFP1

Tags

TAP TAG

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pNH026

Plasmid

#42148

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::TAP TAG

Tags

TAP-Tag

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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