We narrowed to 14,067 results for: LEA

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pSBbi-Puro_LPAR_AKAR-mut

Plasmid

#125207

Purpose

SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter

Depositor

Eric Davis

Insert

LPAR_AKAR-mut

Use

Transposon

Tags

EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

Expression

Mammalian

Mutation

FoxO1 Thr-24 changed to Val rendering this inacti…

Promoter

EF1a/RPBSA

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag D16A+H588A

Plasmid

#109033

Purpose

D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Publication

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-FAK0

Plasmid

#59128

Purpose

Cloning vector for FAK fret sensor without helix

Depositor

Sivaraj Sivaramakrishnan

Publication

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pEMS1210

Plasmid

#29107

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Publication

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple238 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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P23_vanR_Ptec_mCherry

Plasmid

#248114

Purpose

Vanillate switch driving mCherry expression

Depositor

Shrikrishnan Sankaran

Publication

Blanch-Asensio et al J Control Release. 2025 Sep 24;387:11426

Inserts

mCherry red fluorescent protein
VanR repressor

Expression

Bacterial

Promoter

P23 and Ptec flanked by vanO operators

Available since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-EF1a-LibVec

Plasmid

#239591

Purpose

AAV vector for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

Depositor

Andreas Moor

Publication

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

hU6

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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CT691

Plasmid

#233660

Purpose

BLADE (two-input, four-output decoder); Cre excises lox sites to express GFP, Flp excises FRT sites to express iRFP, and the presence of both recombinases is designed to express mRuby.

Depositor

Wilson Wong

Publication

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

BLADE

Expression

Mammalian

Mutation

None

Available since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pISFba1

Plasmid

#226820

Purpose

Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

Depositor

Sheng Yang

Publication

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISFba1

Expression

Bacterial

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-SisTnpB1–ωRNA

Plasmid

#228365

Purpose

Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

Depositor

Tao Liu

Publication

Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

Insert

SisTnpB1

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-R115A/R119A

Plasmid

#80696

Purpose

Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Publication

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-R115A/R119A

Use

AAV

Expression

Mammalian

Mutation

Arg115 and Arg119 both mutated to Ala

Promoter

CBA

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTMEM-PhoCl-Insert

Plasmid

#219674

Purpose

Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

Depositor

Helge Ewers

Publication

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TMEM-PhoCl-rtTA3

Tags

PhoCl

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pInsert-PhoCl-RER

Plasmid

#219676

Purpose

Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

Depositor

Helge Ewers

Publication

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD4mScarlet-PhoCl-RER

Tags

mScarlet and PhoCl

Expression

Mammalian

Promoter

TRE

Available since

June 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRegev_ExonSkip_LandingPad

Plasmid

#204186

Purpose

Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

Depositor

Oded Regev

Publication

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2_8xCTS-ECFP

Plasmid

#200249

Purpose

Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

8xCTS-ECFP

Use

Zebrafish expression

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

Plasmid

#197968

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

Depositor

Eric Fischer

Publication

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

C328S, C331S, L1332V

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-nGFPMNase

Plasmid

#187826

Purpose

express nGFPMNase protein in E coli

Depositor

Yikang Rong

Publication

Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

Insert

nGFPMNASE

Tags

his tag

Expression

Bacterial

Promoter

T7 Promotor

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6_rpoA-C

Plasmid

#190966

Purpose

Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Publication

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Bxb1_jRCaMP1b

Plasmid

#183758

Purpose

Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

Depositor

Richard Davis

Publication

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

jRCaMP1b

Tags

NES

Expression

Mammalian

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Citrine-NLS

Plasmid

#182583

Purpose

Transient expression of Citrine-NLS in plant cell (Nucleus)

Depositor

Yutaka Kodama

Publication

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

NLS

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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mitoZFD_ND2-Left-G1397-N

Plasmid

#180770

Purpose

Expresses mitoZFD_ND2-Left-G1397-N(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Publication

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND2 mutation

Expression

Mammalian

Promoter

pCMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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