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NET30 pEGFP-N2 (815)

Plasmid

#62011

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET30 (MOSPD3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
mTagRFP-T-Fibrillarin-7

Plasmid

#58016

Purpose

Localization: Nucleoli, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Fibrillarin (FBL Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCerulean3-Alpha-5-Integrin-12

Plasmid

#55401

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Alpha-5 Integrin (ITGA5 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105207

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-137

Plasmid

#118555

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Clathrin-15

Plasmid

#55408

Purpose

Localization: Clathrin Vesicles, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Clathrin (CLTB Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pAT-9208

Plasmid

#124221

Purpose

Cloning plasmid for a self-targeting gRNA library

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

self-targeting gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-144

Plasmid

#118528

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE0

Plasmid

#107678

Purpose

Expresses dLbCpf1-BE0 in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE0

Expression

Mammalian

Mutation

D832A/E925/D1148A

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

Plasmid

#164011

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-C-Kbp

Plasmid

#178013

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-C-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-C-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Pa-Kbp

Plasmid

#178014

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Pa-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Pa-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Hv-Kbp

Plasmid

#178015

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Hv-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Hv-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-D-Kbp

Plasmid

#178012

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-D-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-D-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159870

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159867

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mOrange2-EB3-7

Plasmid

#57953

Purpose

Localization: MT End Binding Protein, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

EB3 (MAPRE3 Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBstSK+IRES-V5-IPpoI (mutant)

Plasmid

#206322

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag-mutated IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein (mutant version)

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn11C3S-EGFP

Plasmid

#202747

Purpose

weakly plasma membrane targeted fluorescent protein

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

LYN (1-11) (C3S):EGFP (LYN Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Plasmid

#159861

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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