We narrowed to 2,453 results for: 683

1. mCherry-CRY2-5ptaseOCRL(D523G)

   Plasmid

   #66837

   Purpose

   Expression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   5ptaseOCRL (OCRL Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   D523G

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. TOPO tTR-KRAB-rtTA3-Luc

   Plasmid

   #68449

   Purpose

   TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   

   Use

   Luciferase; Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX4T1-GST-LC3B

   Plasmid

   #216836

   Purpose

   For expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   LC3B (Gly exposed) (MAP1LC3B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   TAC promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. R26TV LSL-TRL

   Plasmid

   #68476

   Purpose

   Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   

   Use

   Cre/Lox, Luciferase, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

   Plasmid

   #192683

   Purpose

   Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PAmCherry1-N1-Lyn11

   Plasmid

   #202683

   Purpose

   Photoactivatable lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   LYN(1-11):PAmCherry1 (LYN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LMX1A-RA

   Plasmid

   #191683

   Purpose

   For knocking p2a-tdTomato into LMX1A locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   LMX1A homology arms and p2a-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHJ425

   Plasmid

   #20683

   Depositor

   Stephen Shaw

   Article

   Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

   Insert

   Ezrin (EZR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Lys 253, 254, 262, 263 to Asn; and change…

   Promoter

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

9. MAC_N_BET1

   Plasmid

   #111683

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   BET1 (BET1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2 mir-1-1 reporter mir16-1 wt

    Plasmid

    #46683

    Purpose

    Expresses wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1 wt (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVRa28_1088

    Plasmid

    #49683

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF28_1088
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBTetBl-CLIP-plk4

    Plasmid

    #136837

    Purpose

    Mammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-plk4 (PLK4 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV hGRN2

    Plasmid

    #213683

    Purpose

    Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

    Depositor

    Thomas Kukar

    Article

    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

    Insert

    human Granulin-2
    

    Use

    AAV

    Tags

    twin-Strep-FLAG

    Expression

    Mutation

    Promoter

    cytomegalovirus enhancer/chicken β-actin promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-MBP-TEVpCS-ADGRB3 CTF-FLAG

    Plasmid

    #217683

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB3 (BAI3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-856

    Promoter

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-DDK_V222A RAD23B

    Plasmid

    #201580

    Purpose

    Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation V222A

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-DDK_F400A RAD23B

    Plasmid

    #201581

    Purpose

    Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation F400A

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-AN-HA_aa 1-420 POLI

    Plasmid

    #201582

    Purpose

    Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-420 of DNA polymerase iota

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-AN-HA_aa 1-542 POLK

    Plasmid

    #201585

    Purpose

    Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    Polymerase kappa (POLK Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-542 of DNA polymerase kap…

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-AN-HA_WT POLI

    Plasmid

    #201440

    Purpose

    Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJM919_WT His POLI

    Plasmid

    #201444

    Purpose

    Low copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    lacIq

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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