Addgene

We narrowed to 2,024 results for: ARP

Showing: 621 - 640 of 2024 results

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEC3079 (p7INT-Pveg_lacI)

Plasmid

#218505

Purpose

integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

lacI (lacI Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pveg

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD

Plasmid

#235574

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-REEP5

Plasmid

#201928

Purpose

PiggyBac Vector expressing KEIMA-REEP5

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

REEP5 (REEP5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN

Plasmid

#175750

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN

Depositor

Wade Harper

Article

Heo et al Mol Cell. 2015 Oct 1;60(1):7-20. doi: 10

Insert

OPTN (OPTN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4 huZAP(L)

Plasmid

#45907

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

ZAP(L) isoform (ZC3HAV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

M202T, L388F, Q701E, T851I (reference NP_064504.2)

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer

Plasmid

#41584

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-RAB5A

Plasmid

#176488

Purpose

For lentiviral transduction of 3FLAG-RAB5A

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

RAB5A (RAB5A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_053

Plasmid

#113591

Purpose

Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

Insert

sgRNA cloning site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-OPTN

Plasmid

#175760

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK TT RBFOX2

Plasmid

#59770

Purpose

doxycycline-inducible expression of untagged human RBFOX2 in mammalian cells

Depositor

Phil Sharp

Article

Jangi et al Genes Dev. 2014 Mar 15;28(6):637-51. doi

Insert

RBFOX2 (RBFOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1709A)

Plasmid

#41588

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

D1709A

Promoter

CMV

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pKH161_FLAG-APEX2-YIPF3 WT

Plasmid

#214980

Purpose

Flag-APEX2 tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3 (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH143_EGFP-YIPF3 LIR mut

Plasmid

#214975

Purpose

GFP tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GFP-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (K70A)

Plasmid

#41589

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K70A

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pKH163_pSmart-mNEON-YIPF4

Plasmid

#214982

Purpose

endogenous mneon tag for YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mNEON with YIPF4 homology (YIPF4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense

Plasmid

#46835

Purpose

Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (E1564A)

Plasmid

#41587

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

E1564A

Promoter

CMV

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only