We narrowed to 8,922 results for: Pol

1. pAAV-hSynI-mCherry-WPRE-hGH poly(A) (Alex_01)

   Plasmid

   #71650

   Purpose

   AAV expression of mCherry in human and mouse neurons

   Depositor

   Mirjana Maletić-Savatić

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynI

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)

   Plasmid

   #71651

   Purpose

   AAV expression of EGFP in human and mouse neurons

   Depositor

   Mirjana Maletić-Savatić

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

   Plasmid

   #218870

   Purpose

   Mammalian expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2(no bind)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   CAG

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

   Plasmid

   #207135

   Purpose

   DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

   Plasmid

   #207136

   Purpose

   DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

   Plasmid

   #208970

   Purpose

   Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-EGFPnls barcode-210-SV40 polyA

   Plasmid

   #190876

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFPnls-barcode210
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1C-no5UTR-Fluc-MtoL-V5-5BoxB-polyA-HhR_AC

   Plasmid

   #148409

   Purpose

   Insect Expression of 5BoxB-polyA-HhR

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   5BoxB-polyA-HhR
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

   Plasmid

   #148446

   Purpose

   Mammalian Expression of 6xbsMS2-PolyA-Malat

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   6xbsMS2-PolyA-Malat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC

    Plasmid

    #148448

    Purpose

    Mammalian Expression of 6xbsMS2-polyA-Malat

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    6xbsMS2-polyA-Malat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

    Plasmid

    #176087

    Purpose

    EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in Lys72 to Ala, and mutation in the PAM…

    Promoter

    CMV

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

    Plasmid

    #174026

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-QF2
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-Flag-Polß(L301R/V303R)-IRES-neo

    Plasmid

    #128664

    Purpose

    mammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

    Plasmid

    #162936

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

    Plasmid

    #162937

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r

    Plasmid

    #162935

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Maltose-binding protein fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-Flag-Polß(L301R/V303R)-IRES-puro

    Plasmid

    #128658

    Purpose

    mammalian expression of Flag-Polß(L301R/V303R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

    Plasmid

    #162932

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 fusion with AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

    Plasmid

    #149350

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-Flag-Polß(L301R/V306R)-IRES-puro

    Plasmid

    #128656

    Purpose

    mammalian expression of Flag-Polß(L301R/V306R) with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V306R

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge