We narrowed to 1,326 results for: TIR

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8s

Plasmid

#169256

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6 (1-373)

Plasmid

#27173

Purpose

For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains only aa 1-373 (please see depositor comm…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pGEM-Nanog

Plasmid

#16351

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Expression

Bacterial

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8f

Plasmid

#192618

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8f

Plasmid

#169258

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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6691 Bicistronic_ires_puro

Plasmid

#64335

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Cdk6-DN-HA

Plasmid

#1869

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pCS2+ Zf GFP-vclb

Plasmid

#185411

Purpose

GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin b (vclb Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf GFP-vcla

Plasmid

#185410

Purpose

GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin a (vcla Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Cdk6-DN

Plasmid

#1867

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
7749 Bicistronic_ires_hygro

Plasmid

#64374

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Venus-Akaluc (neo)

Plasmid

#124701

Purpose

Aka-Luciferase reporter

Depositor

Roland Friedel

Article

Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

Insert

Venus-Akaluciferase

Use

Lentiviral

Promoter

hPGK

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTBK1

Plasmid

#87443

Purpose

express TBK1 in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

TBK1 (Tbk1 Mouse)

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-LC3

Plasmid

#110947

Purpose

Expresses the autophagosome marker LC3 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

mTurquoise2

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8s

Plasmid

#172720

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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