Addgene

We narrowed to 6,093 results for: cat.2

Showing: 621 - 640 of 6093 results

Results per page:

Sort results by:

pKLV2-U6gRNA5(ETS2_5-2)-PGKpuroBFP-W

Plasmid

#211958

Purpose

Express gRNA against ETS2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ETS2 (ETS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

Plasmid

#169181

Purpose

Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

No purification tag and nsp7-nsp8 fusion protein …

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgRipk1_#2-puro

Plasmid

#231983

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(ETV4_5-2)-PGKpuroBFP-W

Plasmid

#211961

Purpose

Express gRNA against ETV4 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ETV4 (ETV4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
1026M=U6-3-gRNA#2[Pol-γ35]

Plasmid

#159675

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#2[Pol-γ35]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
c-myc promoter (TBE 1/2 - wt)

Plasmid

#16595

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (TBE 1/2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
Gln-tRNA-gRNA scaffold for library 1&2

Plasmid

#192367

Purpose

tRNA sequence provider (for checkpoint and immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gln tRNA-gRNA scaffold

Use

For golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSb_init containing the TM287/288 nanobody Nb_TM#2

Plasmid

#132698

Purpose

expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

nanobody Nb_TM#2

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

Plasmid

#224514

Purpose

A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

3xNLS-(G2SG2)2-Cre

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

Plasmid

#112837

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

SH2 + SH3 (C-term) domains of Grb2

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

Plasmid

#227619

Purpose

Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Use

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

deleted aa 2-83

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

Plasmid

#197425

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a 6xHis PreScission SARS-CoV-2 nsp13

Plasmid

#159390

Purpose

Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

Depositor

Tarun Kapoor

Article

Chen et al bioRxiv. 2020 Jul 13. doi: 10.1101/2020.

Insert

N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

Plasmid

#176060

Purpose

Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

K164A and H165A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

Plasmid

#176059

Purpose

Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-RBD-SD1-AVI

Plasmid

#160477

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-SD1

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-RBD-L455RA475RG502R-AVI

Plasmid

#160480

Purpose

Expresses SARS-CoV-2 RBD-L455RA475RG502R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RA475RG502R

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine, Alanine 475 to A…

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI

Plasmid

#160479

Purpose

Expresses SARS-CoV-2 RBD-L455RG496R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RG496R

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine and changed Glyci…

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-RBD-L455RA475R-AVI

Plasmid

#160478

Purpose

Expresses SARS-CoV-2 RBD-L455RA475R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RA475R

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine and changed Alani…

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 puro shTEAD1/3/4-2

Plasmid

#193673

Purpose

Constitutive lentiviral expression of TEAD shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

TEAD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge