We narrowed to 7,456 results for: mme

1. pRDV5 containing Sb-loop

   Plasmid

   #132695

   Purpose

   pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_loop including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPN265NB-LaG16-MNase

   Plasmid

   #170978

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

   Insert

   Glutathione-S-Transferase/LaG16/MNase
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBXNH3CA_MBP

   Plasmid

   #132700

   Purpose

   expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Maltose binding Protein
   

   Tags

   Avi and His

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-NLS-copGFP-EF1-BlastiS

   Plasmid

   #132772

   Purpose

   Spills into the cytoplasm during nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   NLS-copGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

   Plasmid

   #184688

   Purpose

   Mammalian expression, miRFP670nano3

   Depositor

   Vladislav Verkhusha, Axel Nimmerjahn

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3
   

   Use

   AAV

   Promoter

   hSYN

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB-CMV-MCS-Puro dr_cPLA2-mKate2

   Plasmid

   #163844

   Purpose

   Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Tags

   mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Arp2/3_pBIG2abcd

   Plasmid

   #173683

   Purpose

   Insect expression of the 7-subunit Human Arp2/3 complex

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Inserts

   Arp2
   Arp3
   ArpC2
   ArpC3-FLAG
   ArpC4
   ArpC5
   ArpC1
   

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSb_init containing the MBP sybody Sb_MBP#1

   Plasmid

   #132699

   Purpose

   expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   sybody_MBP#1
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+cPLA2-mKate2

   Plasmid

   #162068

   Purpose

   Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Avian, xenopus, zebrafish

   Tags

   mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNubWtXgate

    Plasmid

    #105079

    Purpose

    Yeast mating-based Split Ubiquitin Assay, positive control

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Tags

    NubI

    Expression

    Yeast

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SB_concave

    Plasmid

    #110102

    Purpose

    pBXPHM3 with scaffold Sybody of the concave library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the concave library
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hEF1a, U6

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

    Plasmid

    #187186

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Y90F, V91F

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SB_convex

    Plasmid

    #110104

    Purpose

    pBXPHM3 with scaffold Sybody of the convex library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the convex library
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

    Plasmid

    #194210

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and CJ8421_04975 Rptr2
    

    Expression

    Bacterial

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SB_loop

    Plasmid

    #110103

    Purpose

    pBXPHM3 with scaffold Sybody of the loop library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the loop library
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMUT2-pTcpP-mCherry

    Plasmid

    #192861

    Purpose

    pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
    

    Expression

    Bacterial

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKERep

    Plasmid

    #211826

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CT3G-ERP2-MG

    Plasmid

    #175501

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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