We narrowed to 17,687 results for: BLI

1. pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine

   Plasmid

   #50466

   Purpose

   Gq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-fDIO-GCaMP6s

   Plasmid

   #105714

   Purpose

   Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

   Depositor

   Rylan Larsen

   Has Service

   AAV8

   Insert

   GCaMP6s
   

   Use

   AAV; Flp/frt

   Expression

   Mammalian

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ecoli cpGFP

   Plasmid

   #215713

   Purpose

   Expresses cpGFP in Ecoli

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   circularly permuted GFP

   Promoter

   T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRESN2_GFP_YTA_Champ1_328-354

   Plasmid

   #105639

   Purpose

   Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP

   Depositor

   Thanos Halazonetis

   Insert

   Champ1 (CHAMP1 Human)
   

   Tags

   GFP YTA

   Expression

   Mammalian

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2xMARS

   Plasmid

   #205233

   Purpose

   Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
   

   Tags

   Nuclear Export Sequence and mScarlet-i

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AICSDP-35:AAVS1-mEGFP

   Plasmid

   #91565

   Purpose

   Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

   Depositor

   Allen Institute for Cell Science

   Insert

   AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 7, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. mEmerald-ERBB2-N-18

   Plasmid

   #62755

   Purpose

   Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509

   Depositor

   Michael Davidson

   Insert

   ERBB2 (ERBB2 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Promoter

   TgTUB1 and Toxoplasma U6

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. qMaLioffG

   Plasmid

   #212825

   Purpose

   green fluorescent FLIM indicator for endogenous ATP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al bioRxiv 2023.08.29.555275

   Insert

   qMaLioffG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-pLKO-FOXM1-shRNA-84

    Plasmid

    #89496

    Purpose

    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    H1/TO

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE.puro/TO/Flag/HuR.wt

    Plasmid

    #110346

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tag

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    gag

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac_Dual_SNF2L-opt_p48

    Plasmid

    #102243

    Purpose

    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual

    Depositor

    Joe Landry

    Inserts

    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    

    Expression

    Insect

    Mutation

    codon optimized for expression

    Promoter

    Polyhedrin and p10

    Available Since

    Nov. 1, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET-21a(+)-BMTU UDG-6xHis

    Plasmid

    #172197

    Purpose

    expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions

    Depositor

    Andrea Pauli

    Insert

    BMTU UDG
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-dCas9-TET1-Hygro

    Plasmid

    #104404

    Purpose

    transient expression of dCas9-TET1 fusion protein

    Depositor

    Stephan Beck

    Insert

    TET1 (TET1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-pLKO-FOXM1-shRNA-#3

    Plasmid

    #89500

    Purpose

    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    H1/TO

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50472

    Purpose

    Gs-coupled rM3D-IRES-mCitrine under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    rM3D-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5'Entry-lck-7.4kb

    Plasmid

    #58891

    Purpose

    provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
    

    Use

    Gateway 5' entry clone

    Mutation

    mutated single nucleotide (T-->C, 1874th nucle…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. TCF4 in pENTR

    Plasmid

    #216569

    Purpose

    gateway cloning donor vector containing TCF4

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Gateway entry plasmid

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid

    #183027

    Purpose

    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AICSDP-39:RAB5A-mEGFP

    Plasmid

    #107579

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5A

    Depositor

    Allen Institute for Cell Science

    Insert

    RAB5A Homology Arms with mEGFP-linker (RAB5A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    May 9, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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