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mEmerald-MCM3-C-19

Plasmid

#54165

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM3

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
HA-FOXO3A S253A

Plasmid

#8349

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

S253A

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only
attB53-Pac-TRE-mCherry-attB53

Plasmid

#183611

Purpose

RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

TRE-mCherry

Use

Rmce

Promoter

TRE

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-H3.3-YFP

Plasmid

#8693

Depositor

Kami Ahmad

Article

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3.3A (His3.3A Fly)

Tags

YFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3A T32A

Plasmid

#8348

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

T32A

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only
ETS2_pet28a

Plasmid

#131664

Purpose

Bacterial expression of ETS gene ETS2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETS2 (ETS2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHRi_GPa3b17 TCRα

Plasmid

#187601

Purpose

Lentiviral expression of hTCR alpha (GPa3b17) for low expression

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHRi_GPa3b17 TCRβ

Plasmid

#187602

Purpose

Lentiviral expression of hTCR beta (GPa3b17) for low expression

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 ΔC (1-381)

Plasmid

#62973

Purpose

Expresses HA-tagged rat SOX9 residues 1-383

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA

Expression

Mammalian

Mutation

codes for residues 1-381

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
DNA-CARzeta-IRESpuro

Plasmid

#89345

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta

Use

Lentiviral

Tags

SNAPf tag

Expression

Mammalian

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-PKCB1 K371R

Plasmid

#195230

Purpose

Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PKCB1 (PRKCB Human)

Use

Retroviral

Mutation

K371R

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2a

Plasmid

#157816

Purpose

Fluorescent reporter for histone H2A tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H2A (H2ac13 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGFP FAT

Plasmid

#50517

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase, domain

Tags

GFP

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HA-FOXO3A S315A

Plasmid

#8350

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

S315A

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
KA2628_pGL4-UBCmin-luc2/Tbx3-B'

Plasmid

#124201

Purpose

Luciferase reporter driven by the Tbx3 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Tbx3 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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EHF_pet28a

Plasmid

#131660

Purpose

Bacterial expression of ETS gene EHF

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

EHF (EHF Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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HS-OS-Plk4-mKate2

Plasmid

#105944

Purpose

heat shock inducible transgenesis, non phoshorylable version of Plk4

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Plk4 (Plk4 Mouse)

Tags

mKate2

Expression

Bacterial

Mutation

1002 to 3704 of Plk4/ deletion of S282-S305.

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pACT2-μ3B

Plasmid

#198175

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-3 μ3B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1V5-His Snk/Plk2 T239D

Plasmid

#22220

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Expression

Mammalian

Mutation

single amino acid substitution mutant, threonine …

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only