We narrowed to 87,462 results for: tra
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Plasmid#73797Purposeoptimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only
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ADORA3-Tango
Plasmid#66212PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre
Plasmid#84561PurposepSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loopsDepositorInsertFLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
UseLentiviralTagsFlag, Suntag, oxBFP, AID (auxin-inducible degron)PromoterUbCAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_SBP-mCherry-Golgin84
Plasmid#65304Purposesynchronize trafficking of Golgin-84 from the ER (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
tetO-FUW-eGFP-RHOA-T19N
Plasmid#73082PurposeLentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-T19NDepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-nosT
Plasmid#71272PurposeEntry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertnopaline synthase terminator
UseGatewayAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin1
Plasmid#124852PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
GPRC5D-Tango
Plasmid#66385PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti.tie1.H2B.eYFP.W
Plasmid#51293PurposeLentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoterDepositorAvailable SinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
GPR31-Tango
Plasmid#66351PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Blast DEST ERKKTRmRuby2
Plasmid#90231PurposeLentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmRuby2ExpressionMammalianPromoterPGKAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticExpressionMammalianPromoterhUbCAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6m
Plasmid#40754PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6m
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable SinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1
Plasmid#27099DepositorInsertα-Tubulin K40 acetyltransferase1 (ATAT1 Human)
TagsGFP, S-tag, and TEV siteExpressionMammalianPromoterEF1aAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-VP64
Plasmid#47107PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cellsDepositorInsertdCas9-VP64
UseCRISPRTagsFlag, HA, SV40 NLS, and VP64ExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
p7SL-neo-TET
Plasmid#51285Purposeexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassetteDepositorInsert7SL RNA (RN7SL2 Human)
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoter7SL upstream pollII enhancerAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-dMQ1-EGFP
Plasmid#89637PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG, 6*His, and T2A-EGFPExpressionMammalianMutationC141S, TGC-->TCC; S317A, AGC-->GCCPromoterCMVAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV SYN PSAM4 GlyR IRES EGFP
Plasmid#119742PurposeChemogenetic inhibitor expression plasmidDepositorHas ServiceAAV5InsertPSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
UseAAVExpressionMammalianMutationL131G, Q139L, Y217FPromotersynapsinAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRL838
Plasmid#70680PurposeThis vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.DepositorTypeEmpty backboneUseCre/LoxAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-VGAT-WPRE-pA
Plasmid#39320DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV; RAvailable SinceOct. 9, 2012AvailabilityAcademic Institutions and Nonprofits only