We narrowed to 10,156 results for: ADA

1. ApoE4_47-300_mCh-SspB (pBS1069)

   Plasmid

   #185292

   Purpose

   For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE4_47-300
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ApoE2_47-300_mCh-SspB (pBS1068)

   Plasmid

   #185291

   Purpose

   For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE2_47-300
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLPS2-CAHS2_PARRC::SspB (pBS1043)

   Plasmid

   #185290

   Purpose

   For the mammalian expression of the tardigrade protein CAHS2_PARRC attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS2_PARRC
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLPS1-FUSN:SspB (pBS1041)

   Plasmid

   #185288

   Purpose

   For the mammalian expression of the human protein FUSN attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   FUSN
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJET-62-SFFV-T2A-PuroR-polyA-R11b

   Plasmid

   #179893

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Expression

   Bacterial and Mammalian

   Promoter

   SFFV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJET-64-SFFV-T2A-BlaR-polyA-R11b

   Plasmid

   #179895

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Expression

   Bacterial and Mammalian

   Promoter

   SFFV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R

   Plasmid

   #147394

   Purpose

   Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Nematode, Human)
   

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_SEPT9_i1 S111F-msfGFP

   Plasmid

   #180340

   Purpose

   mammalian expression of human SEPT9_i1 S111F fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 S111F

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_SEPT9_i1 R106W-msfGFP

   Plasmid

   #180339

   Purpose

   mammalian expression of human SEPT9_i1 R106W fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 R106W

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_SEPT9_i1 NCmut1-msfGFP

    Plasmid

    #180329

    Purpose

    mammalian expression of human SEPT9_i1 NCmut1 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 R289A R290A K291A K294A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_SEPT9_i1 D10-25-msfGFP

    Plasmid

    #180334

    Purpose

    mammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 D10-25

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_SEPT9_i1 DN7-msfGFP

    Plasmid

    #180335

    Purpose

    mammalian expression of human SEPT9_i1 DN7 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 DN7

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_SEPT9_i1 Gmut-msfGFP

    Plasmid

    #180331

    Purpose

    mammalian expression of human SEPT9_i1 Gmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 W520A, H530D

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_SEPT9_i1 NCmut2-msfGFP

    Plasmid

    #180330

    Purpose

    mammalian expression of human SEPT9_i1 NCmut2 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 I281D, M288D

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDB-hPER2-SNAP-CD4-bla

    Plasmid

    #179452

    Purpose

    Donor vector to knock in SNAPtag C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    SnapTag
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. S+Q111R

    Plasmid

    #167173

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+Q111R
    ATP1B1
    

    Expression

    Bacterial and Insect

    Mutation

    Changed Q at 111 to R

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. S+N122D

    Plasmid

    #167174

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+N122D
    ATP1B1
    

    Expression

    Bacterial and Insect

    Mutation

    Changed N at 111 to D

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pClgn-Lrrc23-FLAG

    Plasmid

    #179921

    Purpose

    Vector backbone. pClgn-promoter driven expression.

    Depositor

    Masahito Ikawa

    Article

    Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Lrrc23 (Lrrc23 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Stop codon removed

    Promoter

    Calmegin

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pClgn-Lrrc23-1D4

    Plasmid

    #179922

    Purpose

    Vector backbone. pClgn-promoter driven expression.

    Depositor

    Masahito Ikawa

    Article

    Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Lrrc23 (Lrrc23 Mouse)
    

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Stop codon removed

    Promoter

    Calmegin

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNOC_dCas9_sgRNA Td1

    Plasmid

    #176257

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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