We narrowed to 19,557 results for: Mut

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pMGIB-3xFLAG-pontin

Plasmid

#53608

Purpose

Retroviral expression of human pontin

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

pontin (RUVBL1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

pontin CDS mutated to confer resistance to shRNA.…

Promoter

LTR

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-PKM2-H391Y

Plasmid

#122692

Purpose

Express His-tagged PKM2 (H391Y) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

H391Y

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-L26A,F27A

Plasmid

#89084

Purpose

mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

hAR FxxLF mutant (AR Human)

Expression

Mammalian

Mutation

hAR-L26A,F27A

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pLenti-SPA2G231V

Plasmid

#42079

Depositor

Christine Garcia

Article

Maitra et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

Surfactant Protein -A2 (SFTPA2 Human)

Use

Lentiviral; Plenti

Tags

V5

Expression

Bacterial and Mammalian

Mutation

Changed Glycine 231 to Valine

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

Plasmid

#227245

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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mEmerald-Caveolin-N-10

Plasmid

#54026

Purpose

Localization: Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBabe puro H-Ras G12V T35S

Plasmid

#12274

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
GFP-nArgBP2ΔNSE

Plasmid

#74515

Purpose

Express GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2delNSE (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-mCherry-Rheb(C181S)

Plasmid

#192438

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

Lyso-mCherry-Rheb(C181S) (RHEB Human)

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-Rheb(C181S)

Plasmid

#192437

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-F23A

Plasmid

#89089

Purpose

mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

hAR-FxxLF mutant (AR Human)

Expression

Mammalian

Mutation

hAR-F23A

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pHHM.X513-iNicSnFR3b-(V7)

Plasmid

#124881

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3b-(V7)

Tags

HA Tag, His Tag, and NA

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSN838

Plasmid

#184826

Purpose

Expresses human KIF5A(∆exon27) in mammalian cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (∆exon27) (KIF5A Human)

Tags

mScarlet

Expression

Mammalian

Mutation

∆exon27 form

Promoter

CMV

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

CAG promoter

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPong_v2

Plasmid

#47101

Purpose

Expresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.

Depositor

Wayne Parrott

Article

Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

Inserts

mPing
Pong Open Reading Frame 1 (ORF1)
Pong Transposase

Use

Plant expression

Promoter

GmUbi_AP, GmUbi_XS, and no promoter

Available Since

Oct. 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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FgH1tUTG_mup53_Ex5

Plasmid

#85535

Purpose

Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCAD16_CAD_12-490_pvp008

Plasmid

#124842

Purpose

Expresses Aspergillus terreus CAD in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-11, synthetic codon optimiz…

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shGFP

Plasmid

#110939

Purpose

Tet-inducible shRNA targeting GFP

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shGFP

Use

Lentiviral and RNAi

Promoter

H1/TO

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCas9-VRER_2A_GFP

Plasmid

#75475

Purpose

Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

Depositor

Marc Tessier-Lavigne

Article

Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

Insert

Cas9-VRER

Use

CRISPR

Tags

2A_GFP

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

CAG

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-Keap1 S3H4

Plasmid

#21594

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

kelch-like ECH-associated protein 1 (KEAP1 Human)

Tags

HA2

Expression

Mammalian

Mutation

Mutated residues in the beta-sheet S3 and alpha-h…

Available Since

July 8, 2009

Availability

Academic Institutions and Nonprofits only

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