We narrowed to 14,099 results for: cas9

1. pCRISPEY-GAL-Nat-ADE2

   Plasmid

   #232103

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SlugCBE-NNG-P2A-puro

   Plasmid

   #214355

   Purpose

   Expresses SlugCBE-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCBE-NNG and puromycin resistance genes
   

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRISPseq-mCherry-backbone

   Plasmid

   #85708

   Purpose

   Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

   Depositor

   Ido Amit

   Article

   Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE Rims1-GFP KI

   Plasmid

   #131494

   Purpose

   Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Rims1 Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX552-EF1a-DIO-mcherry(with gRNA scaffold)

   Plasmid

   #199581

   Purpose

   Expresses mCherry in a Cre-dependent manner

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   mCherry
   

   Use

   AAV, CRISPR, and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1a intron A and U6

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR1306

   Plasmid

   #111128

   Purpose

   Expresses FANCE.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCE (FANCE Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-NIrD

   Plasmid

   #65010

   Purpose

   NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

   Depositor

   Wensheng Wei

   Article

   Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

   Inserts

   rtTA-MAVS(C)
   2A-linked delta-TK
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Tight-TRE

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

   Plasmid

   #85714

   Purpose

   Telomere-targeting Nme sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVAX_CBI_AcrIIA4

   Plasmid

   #136662

   Purpose

   Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

   Depositor

   Yannick Doyon

   Article

   Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

   Insert

   AcrIIA4
   

   Use

   Synthetic Biology

   Tags

   No tag, No NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. gh90

    Plasmid

    #106761

    Purpose

    expression of gRNA targeting FUT6

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FUT6 (FUT6 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. HR-TERT-promotor

    Plasmid

    #71398

    Purpose

    Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Promoter

    Endogenous TERT promotor

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid

    #74709

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pU6-Decoy

    Plasmid

    #80324

    Purpose

    Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    Decoy sgRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPV-H1-ccdB-mEF1α-RiH

    Plasmid

    #100598

    Purpose

    piggyBac vector for sgRNA cloning

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Type

    Empty backbone

    Use

    Piggybac vector

    Expression

    Mammalian

    Promoter

    Human H1 PolIII promoter, human FTH1 promoter, mo…

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVAX_CBI_AcrIIA2

    Plasmid

    #136661

    Purpose

    Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA2
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. gh39

    Plasmid

    #106713

    Purpose

    expression of gRNA targeting STT3B

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    STT3B (STT3B Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-EN1082_Rad21_STOP

    Plasmid

    #156450

    Purpose

    For transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codon

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    Cas9, RAD21 (Rad21 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. th-P2A-Gal4 donor

    Plasmid

    #65563

    Purpose

    Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th-P2A-Gal4 (th Zebrafish)
    

    Use

    Zebrafish injection

    Promoter

    none

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSP2262

    Plasmid

    #70703

    Purpose

    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    D10A & H557A in SaCas9

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAT-9218

    Plasmid

    #124225

    Purpose

    Bacterial SpCas9 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetR/TetA

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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