We narrowed to 23,486 results for: his

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pLN351 (FuGW-S(YBX1)p-mKate2 )

Plasmid

#105174

Purpose

synthetic promoter YBX1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(YBX1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138734

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLN352 (FuGW-S(Elk1)p-mKate2)

Plasmid

#105175

Purpose

synthetic promoter Elk1 drives mKate2 experssion

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(Elk1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-hDcp2 E147Q, E148Q

Plasmid

#72215

Purpose

bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

His

Expression

Bacterial

Mutation

E147Q, E148Q

Promoter

T7

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCA528 SPASTIN , residues 108-200

Plasmid

#180628

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 STAT3

Plasmid

#67120

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT3 (STAT3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Tags

6x His tag and SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLN381 (FuGW-S(NFY)p-mKate2)

Plasmid

#105179

Purpose

synthetic promoter NFY drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(NFY)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLN431 (FuGW-G8p-mKate2-1Pe )

Plasmid

#105186

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-mKate2-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHIE055 ZF43x3-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138736

Purpose

Integration Vector, assembled with pHIE047 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE052 ZF43x3-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138733

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE056 ZF43x6-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138737

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE057 ZF43x12-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138738

Purpose

Integration Vector, assembled with pHIE049 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE054 ZF43x12-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138735

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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GTH-y

Plasmid

#81105

Purpose

Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCitrine

Use

Cre/Lox

Tags

mCitrine

Expression

Yeast

Mutation

Mutated mCitrine for monomeric isoform (A206K L22…

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pIBA3-SpyCatcherEQ-sfGFP

Plasmid

#107421

Purpose

For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

SpyCatcher

Tags

His tag, StrepII, and superfolder GFP

Expression

Bacterial

Mutation

mutation of glutamate 77 to glutamine

Promoter

Tet

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAimR

Plasmid

#98687

Purpose

Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

Depositor

Rotem Sorek

Article

Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

Insert

AimR

Tags

His-tag

Expression

Bacterial

Promoter

Native

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pETM33_OXSR1_CCT

Plasmid

#178482

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

OXSR1_OXSR1 (OXSR1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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p5B3-T

Plasmid

#92021

Purpose

Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Inserts

TALE targeting lysA with N-terminal TEV protease cleavage site
Tobacco Etch Virus Protease

Use

TALEN

Tags

5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

Mutation

S219V

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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hNedd4-2 CS

Plasmid

#86041

Purpose

full length NeddL3 with C to S mutation with His Xpress tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

Insert

hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)

Tags

His and Xpress

Expression

Mammalian

Mutation

C942S mutation in HECT domain

Promoter

CMV

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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