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pDUET-ctCPR-A13AMO

Plasmid

#20114

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLRD04

Plasmid

#208258

Purpose

Production of ent-kaurene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
blr2150

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-hTICAM1-VenusFlag

Plasmid

#197632

Purpose

Human TRIF protein expression in mammalian cells

Depositor

Haian Fu

Insert

TICAM1 (TICAM1 Human)

Tags

Venus-Flag

Expression

Mammalian

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

Plasmid

#192667

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

Depositor

Jeffrey Miner

Insert

VP64-dCas9-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pB2Hopt

Plasmid

#163830

Purpose

Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B

Depositor

Jerome Fox

Insert

SpecR

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pro1;ProD;pLacZ-Opt

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGART-mCherry

Plasmid

#167164

Purpose

Expresses human GART-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Insert

GART (GART Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRDAX-493 C-Tag GFP[2] L10 Piezo1 EBPF2

Plasmid

#130056

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Piezo1 (Piezo1 Mouse)

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGTRm

Plasmid

#127197

Purpose

pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

tRNA-gRNA PCR template

Use

Template plasmid for generation of golden gate pc…

Promoter

None

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDD373

Plasmid

#137021

Purpose

SapTrap GFP-GLO donor

Depositor

Daniel Dickinson, Bob Goldstein

Insert

C. elegans GLO-optimized GFP

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans GLO-optimized GFP

Expression

Worm

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSpot-Tag-Actin

Plasmid

#117060

Purpose

For mammalian expression of beta-actin with N-terminal Spot-Tag

Depositor

Felix Hartlepp

Insert

actin beta (ACTB Human)

Tags

Spot-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Col2a1:TFPnls-mErt-Cre-Ert

Plasmid

#111152

Purpose

Chondrocyte specific inducible Cre

Depositor

Elly Tanaka

Insert

TFPnls-T2a-mErt-Cre-Ert

Use

Cre/Lox

Mutation

wt

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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mKeima-Red-Tubulin-6

Plasmid

#56020

Purpose

Localization: Microtubules, Excitation: 440, Emission: 620

Depositor

Michael Davidson

Insert

Tubulin (TUBA1B Human)

Tags

mKeima-Red

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
mPlum-ER-3

Plasmid

#55966

Purpose

Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

ER (CALR Human)

Tags

mPlum

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
mPlum-MannII-N-10

Plasmid

#55984

Purpose

Localization: Mannosidase/Golgi, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

MannII (Man2a1 Mouse)

Tags

mPlum

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
mGeos-M-PDHA1-N-10

Plasmid

#57531

Purpose

Localization: Mitochondria, Excitation: 503 / 569, Emission: 514 / 581

Depositor

Michael Davidson

Insert

PDHA1 (PDHA1 Human)

Tags

mGeos-M

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Dronpa-Calnexin-N-14

Plasmid

#57264

Purpose

Localization: Endoplasmic Reticulum, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

NM_001746.3 (CANX Human)

Tags

Dronpa

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PA-mCherry1-PCNA-19-SV40NLS-4

Plasmid

#57192

Purpose

Localization: Nucleus/Replication Origins, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

3x NLS and PA-mCherry1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Dronpa-PDHA1-N-10

Plasmid

#57292

Purpose

Localization: Mitochondria, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

PDHA1 (PDHA1 Human)

Tags

Dronpa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mAmetrine-LaminB1-10

Plasmid

#56547

Purpose

Localization: Nuclear Envelope, Excitation: 406, Emission: 526

Depositor

Michael Davidson

Insert

LaminB1 (LMNB1 Human)

Tags

mAmetrine

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits