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pET28-SUMO-KaiB

Plasmid

#68050

Purpose

Strain: AM5116, Used for protein purification

Depositor

Susan Golden

Publication

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiB

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTarKO

Plasmid

#110540

Purpose

one-step modification of chromosomal locous using targocil/tarO gene in Staphylococcus aureus

Depositor

Suzanne Walker

Publication

Lee et al ACS Infect Dis. 2018 Mar 23. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Targeting vector for locus of …

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL

Plasmid

#101002

Purpose

Construct for fusion expression of GeNL reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Publication

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FPPS

Plasmid

#108952

Purpose

heterologous expression of FPPS of G. stearothermophilus in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Farnesyldiphosphat synthase (FPPS)

Tags

His6-Tag

Expression

Bacterial

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-iCre (JDW 921)

Plasmid

#224519

Purpose

A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP-P2A-iCre

Use

Cre/Lox

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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IDG_CACNA2D3_OE_1

Plasmid

#161683

Purpose

Overexpresses CACNA2D3

Depositor

Mike McManus

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CACNA2D3 (CACNA2D3 Human)

Tags

V5

Expression

Mammalian

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_TMC5_OE_1

Plasmid

#161621

Purpose

Overexpresses TMC5

Depositor

Mike McManus

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TMC5 (TMC5 Human)

Tags

V5

Expression

Mammalian

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

Plasmid

#170222

Purpose

Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Publication

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

R24C

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pNM3725

Plasmid

#154113

Purpose

UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

UAS 11X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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C1ORF43-GFP

Plasmid

#130879

Purpose

Expresses human C1ORF43 with C-terminal GFP tag

Depositor

Michael Bassik

Publication

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

Plasmid

#87906

Purpose

sgRNA targeting GFP

Depositor

Nicole Déglon

Publication

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgGFP1, sgGFP2, sgGFP3

Expression

Mammalian

Promoter

H1, U6, 7SK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB3047(gRNA XII-1)

Plasmid

#73289

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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shCDK4/6(2)

Plasmid

#73555

Purpose

vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

Plasmid

#52539

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Expression

Mammalian

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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mCitrine-Peroxisomes-2

Plasmid

#54672

Purpose

Localization: Peroxisomes, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCitrine-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pLKO-shAUF1 hairpin 1

Plasmid

#52921

Purpose

expresses a hairpin specific for human AUF1

Depositor

Sheila Stewart

Publication

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting AUF1 (HNRNPD Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
NEDD4L-C2

Plasmid

#25615

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

NEDD4L-C2 (NEDD4L Human)

Tags

His

Expression

Bacterial

Mutation

Contains C2 domain

Available since

Aug. 31, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pMM1157

Plasmid

#112534

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Depositor

Timothy Lu

Publication

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
luxCDABE

Expression

Bacterial

Promoter

PL(HrtO) and ProD

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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AiP14478 - pAAV-AiE0456m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4478)

Plasmid

#229923

Purpose

AiE0456m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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