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We narrowed to 17,881 results for: puromycin

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Showing: 6501 - 6520 of 17881 results

  5. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139097-plasmid-map-sequence-id-273896

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  6. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

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  9. pR26-mBMP7

    Plasmid
    #127374
    Purpose
    Allows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Publication
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMP7 (Bmp7 Mouse)
    Use
    CRISPR and Mouse Targeting
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127374-plasmid-map-sequence-id-247972

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  10. p663-UBC-TurboID-V5-BRSK2_IDG-K

    Plasmid
    #135272
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-BRSK2 (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135272-plasmid-map-sequence-id-269112

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  11. BII-ChPtW-smURFP

    Plasmid
    #133399
    Purpose
    Piggybac vector for constitutive expression of smURFP-NLS
    Depositor
    Blair Madison
    Insert
    smURFP-NLS
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133399-plasmid-map-sequence-id-268584

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  12. p663-UBC-V5-BRSK1_IDG-K

    Plasmid
    #135270
    Purpose
    Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-BRSK1 (BRSK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135270-plasmid-map-sequence-id-268548

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  13. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdTomato-NLS
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  16. pUC-STITCH

    Plasmid
    #129535
    Purpose
    STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.
    Depositor
    Hideyuki Okano
    Publication
    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STITCH
    Use
    CRISPR; Donor template
    Available since
    Dec. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    129535-plasmid-map-sequence-id-265185

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  17. GWF180

    Plasmid
    #133607
    Purpose
    CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9
    Depositor
    Dustin Maly
    Publication
    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
    Expression
    Mammalian
    Available since
    Oct. 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    133607-plasmid-map-sequence-id-261658

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Showing: 6501 - 6520 of 17881 results