We narrowed to 13,831 results for: sequence

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pTRE3G-Fluc-DR

Plasmid

#242434

Purpose

Firefly Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the TRE3G promoter.

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Tags

CasRx Direct Repeat

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCITF-KCC2

Plasmid

#61404

Purpose

Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

Depositor

Franck Polleux

Article

Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

Insert

KCC2 (SLC12A5 Human)

Tags

IRES-tdTomato-Flag

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Article

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18/promEco/Lysostaphin

Plasmid

#125765

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK-Sa1 promoter

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV-EF1s HA.NLS.Sce(opt)

Plasmid

#31479

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV/Ef1s HA NLS I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

"opt" indicates the coding sequence has…

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Mutation

Deleted the partial 3'ribozyme sequence in T…

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Article

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AKAR4

Plasmid

#61620

Purpose

Lipid raft-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

Lyn-AKAR4

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-mCherry

Plasmid

#113089

Purpose

General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-mCherry

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-GFP

Plasmid

#112851

Purpose

General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-GFP

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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LRT_RTctrl

Plasmid

#214074

Purpose

Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LRT

Plasmid

#214071

Purpose

Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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ARTX_ctrl

Plasmid

#214065

Purpose

Fluorescent reporter system, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Remnant of linker sequence present at the end of …

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGFAP-TetOn3G

Plasmid

#133728

Purpose

Plasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G

Use

AAV

Expression

Mammalian

Promoter

pGFAP

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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AICSDP-19:LAMP1-mEGFP

Plasmid

#101782

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

Depositor

Allen Institute for Cell Science

Insert

LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Available Since

Nov. 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

Plasmid

#219537

Purpose

a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
NLS-tdMCP-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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ART_RTctrl

Plasmid

#214066

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI3

Plasmid

#42527

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

WIPI3 (WDR45B Human)

Tags

GST-TEV

Expression

Mammalian

Mutation

gene sequence is codon optimized for expression i…

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pTOX4

Plasmid

#127449

Purpose

Encodes YhaV toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

yhaV toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-TagBFP

Plasmid

#113086

Purpose

General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-TagBFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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