We narrowed to 14,352 results for: Cas9

Showing: 6541 - 6560 of 14352 results

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pCAS_gpyrG1

Plasmid

#90276

Purpose

(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

Depositor

Michael Sauer

Publication

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg1)

Use

A. niger

Expression

Bacterial

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR1003

Plasmid

#71821

Purpose

Express Streptococcus pyogenes dCas9 (D10A, H840A)

Depositor

Jacob Corn

Publication

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)

Use

CRISPR

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ138A

Plasmid

#69280

Purpose

Golden Gate entry vector; 8th gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ137A

Plasmid

#69279

Purpose

Golden Gate entry vector;7th gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ147

Plasmid

#69299

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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sgRNA3_MYOD1

Plasmid

#64138

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA3_IL1RN

Plasmid

#64151

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-HaloTag-Puromycin

Plasmid

#167208

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

Depositor

Oscar Perez

Publication

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

HaloTag

Expression

Mammalian

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT007

Plasmid

#171634

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Publication

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE Doc2A-GFP KI

Plasmid

#131478

Purpose

Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Doc2a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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CP1012-CBEmax

Plasmid

#119801

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rApobec1-nSpCas9 (CP1012) -UGI-UGI

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MSP2279

Plasmid

#70706

Purpose

Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Publication

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

D10A and H557A in SaCas9

Promoter

T7

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199481

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199482

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-i53

Plasmid

#119002

Purpose

Mammalian expression of i53 (53BP1) inhibitor and BFP

Depositor

Ralf Kuehn

Publication

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

i53

Expression

Mammalian

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-EMX1

Plasmid

#140581

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Publication

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA EMX1

Use

CRISPR

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8742

Plasmid

#126908

Purpose

Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

pGPD1 and pFBA1

Use

Synthetic Biology

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

Plasmid

#121448

Purpose

To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

Depositor

Brian Chen

Publication

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RFPnols-PQR-ZeocinR (ACTB Human)

Use

CRISPR

Expression

Mammalian

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCF708

Plasmid

#121685

Purpose

EF1a-dTEV-protease-mTagBFP2

Depositor

David Savage

Publication

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

TEV protease

Use

Lentiviral

Promoter

EF-1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD3-ovoD1

Plasmid

#111142

Purpose

Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

Insert

pCFD3-ovoD1 (ovo Fly)

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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