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pLKO.1 p18 shRNA

Plasmid

#26631

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

p18 shRNA (LAMTOR1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
CRY2olig-mCh-CLC

Plasmid

#60035

Purpose

Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2-mCh-Clathrin Light Chain (CRY2 Mustard Weed, Mouse)

Expression

Mammalian

Mutation

M354I, E490G CRY2, A9D*

Promoter

CMV

Available Since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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DHFR-mNeon-GluA1

Plasmid

#173009

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-mNeon-GluA1

Tags

DHFR and mNeon

Expression

Mammalian

Promoter

Chicken beta actin (CAG)

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-FGL

Plasmid

#190234

Purpose

AAV vector, high-level expression of myrGFP with a somatodendritic-targeting signal (FGL) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

LDLR C-terminal sequence and myristoylation signa…

Expression

Mammalian

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCD565

Plasmid

#153027

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hMBOAT7-3xFLAG

Plasmid

#165474

Purpose

Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

Membrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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phage - ubc - nls - 2xmcp - egfp - BirA*

Plasmid

#131132

Purpose

BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Depositor

Ralf Jansen

Article

Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

Insert

NLS-HA-2xMCP-EGFP-BirA*

Use

Lentiviral

Tags

NLS, HA

Expression

Mammalian

Mutation

BirA* = BIrA >R118G

Promoter

human ubiquitin C (UBC)

Available Since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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TfR-moxGFP-DHFR

Plasmid

#173012

Purpose

For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

TfR-moxGFP-DHFR

Tags

DHFR and moxGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-beta1(617-949)-GFP

Plasmid

#100732

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP1B1 (AP1B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Stop-EGFP (MSE)

Plasmid

#154469

Purpose

MSCV-Stop-EGFP-pgk-Neo (retroviral construct containing the RFP-based translational stop cassette)

Depositor

Cornelius Miething

Article

Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

Plasmid

#140535

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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PGK ARF6(Q67L)-CFP

Plasmid

#200428

Purpose

Mammalian expression of Q67L mutation in ARF6

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

ARF6 (ARF6 Human)

Tags

CFP

Expression

Mammalian

Mutation

Q67L

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 MP1 shRNA

Plasmid

#26632

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

MP1 shRNA (LAMTOR3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pSUMO His6 SUMO PYL1 (36-211)

Plasmid

#177843

Purpose

Bacterial Expression of PYL1

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 1 (PYL1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FAM20C:V5

Plasmid

#63584

Purpose

FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.

Depositor

Kenneth Irvine

Article

Ishikawa et al PLoS One. 2012;7(8):e42988. doi: 10.1371

Insert

FAM20C (Fam20c Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pUAST-attB-3xHA-V5-RpL10Ab

Plasmid

#125223

Purpose

3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

3x HA, V5

Expression

Insect

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSLIK-Hygro-Myc-hAgo2

Plasmid

#60234

Purpose

Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

Insert

Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pJZC620

Plasmid

#62282

Purpose

Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
PCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Nuclease activity has been inactivated by mutatio…

Promoter

pAdh and pTdh3

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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PDZb-beta2(616-951)-mCherry

Plasmid

#100736

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

PDZb and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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hGATA-1 K137R

Plasmid

#118353

Purpose

This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K137R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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