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We narrowed to 18,348 results for: multi

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Showing: 6541 - 6560 of 18348 results

  5. pACDura3

    Plasmid
    #138711
    Purpose
    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
    Depositor
    Víctor de Lorenzo
    Publication
    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21
    Insert
    ura3 gene (URA3 Budding Yeast)
    Expression
    Bacterial
    Mutation
    optimized ura3 gene for expression in Escherichia…
    Promoter
    pEM7 promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138711-plasmid-map-sequence-id-311288

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  7. pCMV_mCherry_2A_HA_2xMS2-VP64

    Plasmid
    #68419
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    MS2: V75E/A81G
    Promoter
    CMV
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    68419-plasmid-map-sequence-id-120197

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  8. GRM5-DuET

    Plasmid
    #213303
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM5 (GRM5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213303-plasmid-map-sequence-id-431813

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  9. dpYPQ230-D156R-CBE4

    Plasmid
    #195369
    Purpose
    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    hA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
    Use
    CRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…
    Expression
    Plant
    Promoter
    ZmUBI1
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195369-plasmid-map-sequence-id-392981

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  10. pALS099 (pGEX-2TP-cTevHis-DEST)

    Plasmid
    #139787
    Purpose
    Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis
    Depositor
    Jeannie Lee, Arneet Saltzman
    Publication
    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:
    Type
    Empty backbone
    Tags
    6xHis (cleavable by TEV protease) and GST (cleava…
    Expression
    Bacterial
    Available since
    April 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139787-plasmid-map-sequence-id-325052

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  14. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Publication
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

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  16. pRS416gT-GalL-Cas9

    Plasmid
    #79903
    Purpose
    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
    Depositor
    Ronald Davis
    Publication
    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw
    Inserts
    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GPM1 promoter, GalL, and RPR1 promoter with TetO …
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    79903-plasmid-map-sequence-id-157872

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  20. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid
    #178429
    Purpose
    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950 Glu66→Thr
    Optimized G
    EGFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Promoter
    hSyn
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178429-plasmid-map-sequence-id-354737

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Showing: 6541 - 6560 of 18348 results