We narrowed to 14,099 results for: cas9

1. pX552 EF1a DIO Chronos GFP_Vgat gRNA

   Plasmid

   #215277

   Purpose

   Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Vgat gRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

   Plasmid

   #215278

   Purpose

   Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Vgat control gRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

   Plasmid

   #215281

   Purpose

   Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Grin1 gRNA
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEL670

   Plasmid

   #196818

   Purpose

   LrCas9 cytosine base editing plasmid in rice

   Depositor

   Yong Zhang

   Article

   Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   ZmUbi

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEL671

   Plasmid

   #196819

   Purpose

   LrCas9 cytosine base editing plasmid in wheat

   Depositor

   Yong Zhang

   Article

   Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   ZmUbi

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEL672

   Plasmid

   #196820

   Purpose

   LrCas9 adenine base editing plasmid V1.0 in rice

   Depositor

   Yong Zhang

   Article

   Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   ZmUbi

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEL673

   Plasmid

   #196821

   Purpose

   LrCas9 adenine base editing plasmid V2.0 in rice

   Depositor

   Yong Zhang

   Article

   Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   ZmUbi

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Immune checkpoint (library 2) Backbone

   Plasmid

   #192363

   Purpose

   Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. T cell activation (library 1) Backbone

   Plasmid

   #192364

   Purpose

   Plasmid vector for in vitro Jurkat activation screening (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gly-tRNA-gRNA scaffold for library 2

    Plasmid

    #192365

    Purpose

    tRNA sequence provider (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gly tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pro-tRNA-gRNA scaffold for library 2

    Plasmid

    #192366

    Purpose

    tRNA sequence provider (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Gly-tRNA-gRNA scaffold for library 1

    Plasmid

    #192368

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Gly tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pro-tRNA-gRNA scaffold for library 1

    Plasmid

    #192369

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPQD1CB0001

    Plasmid

    #185625

    Purpose

    MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGG422_UV5

    Plasmid

    #165607

    Purpose

    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    D1332K and R1333G mutations in SpCas9

    Promoter

    lacUV5 driving Cas9 KG and UV5 driving sgRNA

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti_stgRNA-Puro

    Plasmid

    #164124

    Purpose

    Backbone for lentiviral stgRNA library construction (library 1 and 2)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAT-9251

    Plasmid

    #124226

    Purpose

    Bacterial SpCas9-HF1 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetR/TetA

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-BRCA1-RNF169UBD

    Plasmid

    #119009

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-BRCA1-Rad18UBD

    Plasmid

    #119008

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

    Plasmid

    #133795

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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