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Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterARG8Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterGNP1Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vinculin-Clover-CC-S7-mRuby2
Plasmid#233236PurposeVinculin FRET tension sensor with coiled-coil S7 domain no vinculin force controlDepositorInsertVinculin-Clover-CC-S7-mRuby2
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vinculin-Clover-CC-S4-mRuby2
Plasmid#233235PurposeVinculin FRET tension sensor with coiled-coil S4 domain no vinculin force controlDepositorInsertVinculin-Clover-CC-S4-mRuby2
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vinculin-Clover-CC-S2-mRuby2
Plasmid#233234PurposeVinculin FRET tension sensor with coiled-coil S2 domain no vinculin force controlDepositorInsertVinculin-Clover-CC-S2-mRuby2
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Bxb1-I87L, A369P, E434G
Plasmid#232750PurposeIVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSExpressionMutationI87L, A369P, E434GPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-His-PDCD1LG2
Plasmid#232660Purposetransient expresses PD-L2 in mammalian cellsDepositorInsertPDCD1LG2 (PDCD1LG2 Human)
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Myc-KLF16
Plasmid#232655Purposetransient expresses KLF16 in mammalian cellsDepositorInsertKLF16 (KLF16 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-His-VTCN1
Plasmid#232664Purposetransient expresses VTCN1 in mammalian cellsDepositorInsertVTCN1 (VTCN1 Human)
UseTagsHis and His (retained after signal peptide cleava…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG-ITPKA-mScarlet-I
Plasmid#229778PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporterDepositorInsertmScarlet-I
UseSynthetic BiologyTagsITPKAExpressionMammalianMutationPromoterCAGAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vinculin-Clover-CC-Mad-mRuby2
Plasmid#233239PurposeVinculin FRET tension sensor with coiled-coil Mad domain no vinculin force controlDepositorInsertVinculin-Clover-CC-Mad-mRuby2
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vinculin-Clover-CC-S0-mRuby2
Plasmid#233233PurposeVinculin FRET tension sensor with coiled-coil S0 domain no vinculin force controlDepositorInsertVinculin-Clover-CC-S0-mRuby2
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Convergent
Plasmid#229790PurposeBxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent
Plasmid#229800PurposeBxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1aDepositorInsertmGreenLantern; mRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Divergent
Plasmid#229791PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+) pCopA RBS30 sfgfp
Plasmid#226371PurposePlasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30DepositorInsertsfGFP
UseTags6x HisTagExpressionBacterialMutationPromotercopA promoter (pCopA) and RBS30 regulationAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
hnRNPAB X2-IDR
Plasmid#216517PurposeExpresses MBP-tagged intrinsically disordered region of hnRNPAB X2DepositorInserthnRNPAB X2-IDR
UseTags6xHis tags-MBPExpressionBacterialMutationdeleted amino acids 2-225PromoterAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX 318-320A
Plasmid#192060PurposeExpression vector for SOX (ORF37) 318-320ADepositorInsertSOX (ORF37)
UseTagsExpressionInsectMutation318-320APromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMDC Renilla Luc SRE
Plasmid#191657PurposeExpressed Renilla Luciferase with SOX resistance elementDepositorInsertRenilla Luciferase SRE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
SOX D221A/E244A/K246A pACEmam1
Plasmid#192050PurposeExpresses a 3X catalytic mutant of SOX (ORF37)DepositorInsertSOX (3X catalytic mutant)
UseUnspecifiedTagsExpressionMutation3X catalytic mutant D221A/E244A/K246APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) K246A
Plasmid#192051PurposeExpresses a catalytic mutant of SOX (K246A)DepositorInsertSOX (K246A)
UseUnspecifiedTagsExpressionMutationCatalytic mutant K246APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) K250A
Plasmid#192052PurposeExpresses a DNA binding mutant K250A (ORF37)DepositorInsertSOX (K250A)
UseUnspecifiedTagsExpressionMutationDNA binding mutant K250APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) 318-320A
Plasmid#192054PurposeExpresses a mutant of SOX 318-320ADepositorInsertSOX (318-320A)
UseUnspecifiedTagsExpressionMutationSOX 318-320APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-H-V5-His
Plasmid#222850PurposeE. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-H
UseTagsV5-HisExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
WDV
Plasmid#226983PurposeExpresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experimentsDepositorInsertWDV (repA )
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-A-V5-His
Plasmid#222849PurposeE. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-A
UseTagsV5-HisExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-PRM-wasabi
Plasmid#195554PurposeTemperature-dependent expression of mWasabi reporter with transcription activation by TcI38DepositorInsertsmWasabi
TcI38
UseTags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD043
Plasmid#212913PurposeEncodes the S. cerevisiae 111 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSCW4 (SCW4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD084
Plasmid#212921PurposeEncodes the HA-tagged S. cerevisiae Tip1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertTIP1 (TIP1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD080
Plasmid#212917PurposeEncodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA1 (AGA1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD001
Plasmid#212898PurposeEncodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD008
Plasmid#212905PurposeEncodes S. cerevisiae PLB1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertPLB1 (PLB1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD006
Plasmid#212903PurposeEncodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD083
Plasmid#212920PurposeEncodes the HA-tagged S. cerevisiae Sed1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSED1 (SED1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD082
Plasmid#212919PurposeEncodes the HA-tagged S. cerevisiae Cwp2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertCWP2 (CWP2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD003
Plasmid#212900PurposeEncodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertECM14 (ECM14 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD002
Plasmid#212899PurposeEncodes S.cerevisiae CRH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertCRH1 (CRH1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD007
Plasmid#212904PurposeEncodes S. cerevisiae OST1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertOST1 (OST1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD041
Plasmid#212911PurposeEncodes the S. cerevisiae YAR066W Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertYAR066W (YAR066W Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD023
Plasmid#212909PurposeEncodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only