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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

G120C

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-Flag-ZO-2

Plasmid

#27420

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
SERK2 P5_pECIA14

Plasmid

#114768

Purpose

Prey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK2 P5_pECIA2

Plasmid

#114968

Purpose

Bait vector SERK2 P5_pECIA2 should be used with prey vector SERK2 P5_pECIA14.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG

Plasmid

#15938

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMSCV-Myc-Caspase-11_humanized-mutant

Plasmid

#214316

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Mutation

KLS288YKT_KASIHS348TPRAKA

Promoter

MESV

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-11_humanized-mutant

Plasmid

#214315

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

N152K_KLS288YKT_KASIHS348TPRAKA

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pC0078 RESCUE

Plasmid

#130661

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dRanCas13b-ADAR2dd (RESCUE)

Expression

Mammalian

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

Plasmid

#186991

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, non-targeting crRNA guide

Use

AAV

Mutation

r1045-gggs-r1122 deletion

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_TRET_Ngn2‐2A‐Ngn1

Plasmid

#61471

Purpose

3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

Depositor

Ron Weiss

Publication

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (Neurog2 Mouse)
Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pC0079 RESCUE-S

Plasmid

#130662

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dRanCas13b-ADAR2dd

Expression

Mammalian

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFAB3869

Plasmid

#47842

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 11 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Publication

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9

Expression

Mammalian

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p3XFLAG-CMV-14-WDR5

Plasmid

#59974

Purpose

Mammalian Expression of WDR5-FLAG

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WDR5 (WDR5 Human)

Tags

FLAG

Expression

Mammalian

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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EGFP-Rab11a-7

Plasmid

#56444

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Publication

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab11a (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Publication

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Publication

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBABEhygro-Flag-ZO-2

Plasmid

#27473

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pEG BacMam C term 3C eGFP StrepII

Plasmid

#160686

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

3C eGFP StrepII

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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