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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,422 results for: FRI

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Showing: 641 - 660 of 3422 results

  3. pEK42

    Plasmid
    #214958
    Purpose
    Endogenous tagging of B. dendrobatidis GAPDH with a red fluorescent protein, hygromycin B selection
    Depositor
    Lillian Fritz-Laylin
    Article
    Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122
    Inserts
    BdGAPDH_LHA2-G4S-HsmRuby3-ScADH1ter
    SpH2Bpro-Hshph-BdGAPDH_RHA2
    Use
    Bd gene targeting vector
    Expression
    Bacterial
    Promoter
    SpH2Bpro
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    214958-plasmid-map-sequence-id-426037

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  4. pEK48

    Plasmid
    #214964
    Purpose
    Endogenous tagging of B. dendrobatidis Myo17D with a red fluorescent protein, hygromycin B selection
    Depositor
    Lillian Fritz-Laylin
    Article
    Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122
    Inserts
    BdMyo17D_LHA-G4S-HsmRuby3-ScADH1ter
    SpH2Bpro-Hshph-BdMyo17D_RHA
    Use
    Bd gene targeting vector
    Expression
    Bacterial
    Promoter
    SpH2Bpro
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    214964-plasmid-map-sequence-id-426034

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  7. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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  12. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  15. KCC2-pHext

    Plasmid
    #104076
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Tags
    pHluorine tag inserted in the second putative tra…
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104076-plasmid-map-sequence-id-199360

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  17. pHalo2X-mMYO7A-RK/AA

    Plasmid
    #251426
    Purpose
    Mammalian expression vector for expressing a HaloTag-fused mouse MYO7A mutant that destabilizes the autoinhibitory conformation.
    Depositor
    Thomas Friedman, Takushi Miyoshi
    Article
    Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:
    Insert
    Myosin VIIa (RK/AA) (Myo7a Mouse)
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Two missense mutations in the RGSK motif of the s…
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251426-plasmid-map-sequence-id-501427

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Showing: 641 - 660 of 3422 results