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pINDUCER dCas9-TET1CD

Plasmid

#101921

Purpose

The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

Depositor

Danwei Huangfu

Publication

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

dCas9-TET1CD

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM4723

Plasmid

#48015

Purpose

Please see depositor comments section.

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

30 Articles

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pH2B-miRFP703

Plasmid

#80001

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMXs-EGFP-Rheb-IP

Plasmid

#13831

Depositor

Shinya Yamanaka

Publication

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pX330 spCas9-mSA

Plasmid

#113096

Purpose

pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

Synthetic Biology

Tags

MSA (monomeric streptavidin

Mutation

none

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV5B-Flag-Smurf1 C699A

Plasmid

#11753

Depositor

Jeff Wrana

Publication

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

C699A: catalytically inactive

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pB33eCPX

Plasmid

#23336

Depositor

Patrick Daugherty

Publication

Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

Insert

Enhanced Circularly Permuted Outer Membrane Protein X

Expression

Bacterial

Available since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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YFP-NavII-III

Plasmid

#26056

Depositor

Matthew Grubb

Publication

Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Insert

Nav1.2 (Scn2a Rat)

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Available since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Publication

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Promoter

beta-actin, sp6, t7

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Publication

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Mito-mEosEM

Plasmid

#132706

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Publication

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mitochondria targeting sequence

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag TSC2 wt

Plasmid

#12132

Depositor

Cheryl Walker

Publication

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PM-FRB-mRFP-T2A-FKBP-5-ptase

Plasmid

#40896

Depositor

Peter Varnai

Publication

Toth et al J Cell Sci. 2012 May 1;125(Pt 9):2185-97

Insert

PM-FRB-mRFP-T2A-FKBP-5-ptase

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.2/v5-DEST hGlut2

Plasmid

#18086

Depositor

Wolf Frommer

Publication

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut2 (SLC2A2 Human)

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Publication

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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TfR-sfGFP-myc tag-SpyCatcher003

Plasmid

#133451

Purpose

Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

TfR-sfGFP-myc tag-SpyCatcher003

Tags

GSSGS and myc tag

Expression

Mammalian

Mutation

Contains C20 and A23 mutations that improve plasm…

Promoter

CMV

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Publication

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEGFP VAMP4

Plasmid

#42313

Depositor

Thierry Galli

Publication

Mallard et al J Cell Biol. 2002 Feb 18;156(4):653-64.

Insert

VAMP 4 (VAMP4 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LL - hOCT4i -2

Plasmid

#12197

Depositor

George Daley

Publication

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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