We narrowed to 34,429 results for: emb

1. TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

   Plasmid

   #201968

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   W255A,N262E,K266E

   Available Since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFR093

   Plasmid

   #134149

   Purpose

   Expression of CENP-T

   Depositor

   Iain Cheeseman

   Article

   Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

   Insert

   CENP-T (CENPT Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 1-375 with T85D mutation

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-cMSP1D1

   Plasmid

   #199768

   Purpose

   Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP1D1
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-cMSPdH5

   Plasmid

   #199771

   Purpose

   Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH5
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-cMSPdH4-6

   Plasmid

   #199766

   Purpose

   Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH46
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-cMSPdH45

   Plasmid

   #199767

   Purpose

   Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH45
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 Blast NT shRNA

   Plasmid

   #123306

   Purpose

   Expression of a non-targeting shRNA

   Depositor

   Giovanni Tonon

   Article

   Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

   Insert

   Non-targeting shRNA
   

   Use

   Lentiviral and RNAi

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. C1-MPAct-mCherry

   Plasmid

   #155222

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. C1-MPAct-mRuby3

    Plasmid

    #155221

    Purpose

    Monitor changes in the density of membrane-proximal F-actin (MPA)

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    

    Tags

    mRuby3

    Expression

    Mammalian

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. C1-MPAct-mCitrine

    Plasmid

    #155220

    Purpose

    Monitor changes in the density of membrane-proximal F-actin (MPA)

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-mCherry2-sgRNA (empty)

    Plasmid

    #198330

    Purpose

    Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    U6-sgRNA(F+E) empty
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRAPa-3xFLAG-pCDNA

    Plasmid

    #201963

    Purpose

    Mammalian expression of Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Available Since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML2(−)WF

    Plasmid

    #108337

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pML2(+)BR

    Plasmid

    #108336

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pML2(+)WF

    Plasmid

    #108333

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML2(+)BF

    Plasmid

    #108334

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML2(+)WR

    Plasmid

    #108335

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML2(−)BF

    Plasmid

    #108338

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML2(−)WR

    Plasmid

    #108339

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge